IGEM:IMPERIAL/2009/Encapsulation/Biobricks

Biobricks

Module 1

Module 1 biobricks

Module 2

Module 2 biobricks

Module 3

Module 3 biobricks

Biobricks from the registry

Table 1. Biobricks we need from the registry

Promoters:

Module	Part	BB #	Kit	Well #	Plasmid	Resistance	Copy #	Link
M1	Inducible (IPTG)	BBa_R0011	KP1 2009	6G	pSB1A2	A	100-300	[1]
M2	Inducible (cAMP)	BBa_K118011	KP2 2009	18D	pSB1A2	A	100-300	[2]
M3	Constitutive	BBa_J23114	KP1 2009	20I	BBa_J61002	A	15-20 (based on pBR322 ORI)	Constitutive promoter
M3	Constitutive	BBa_J23103	KP1 2009	18I	BBa_J61002	A	15-20 (based on pBR322 ORI)	Constitutive promoter
M3	Repressible (lambda cI)	BBa_R0051	KP1 2009	6K	pSB1A2	A	100-300	Promoter (lambda cI regulated)
M3-Testing	Inducible(Arabinose)	BBa_I0500	KP1 2009	14N	pSB2K3	K	Variable – IPTG results in high copy # (++100 per cell)	pBAD Promoter

Ribosome Binding Sites

Terminators

Module	Part	BB #	Kit	Well #	Plasmid	Resistance	Copy #	Link
1,2,3	RBS	BBa_B0034	KP1 2009	2M	pSB1A2	A	100-300	RBS

Composite Parts

Module	Part	BB #	Kit	Well #	Plasmid	Resistance	Copy #	Link
1,2,3	Double terminator	BBa_B0015	KP1 2009	23L	pSB1AK3	AK	100-300	Double terminator

Module	Part	BB #	Kit	Well #	Plasmid	Resistance	Copy #	Link
M1,M2,M3 (Testing)	GFP generator	BBa_E0240	KP1 2009	12M	pSB1A2	A	100-300	GFP generator
M1,M2,M3 (Testing)	Variable input (testing)	BBa_F2620	KP2 2009 BB_Barry Box 1	6E	pSB1A2	A	100-300	Variable input
M3	HARVARD - cI QPI with high promoter (heat sensitive)	BBa K098995	KP3 2009	1E	pSB1A2	A	100-300	cI QPI with high promoter

Biobricks from MrGene

Table 1. Biobricks we need to synthesise.

Biobricks

Gene	NCBI Ref	Nucleotide accession#	Organism	Xbal & SpeI sites	Start?	Stop?	ORF	References
PAH	5053	PAH #	Homo Sapiens	1 and 0	Yes	Yes	1359bp (+1)	PAH
Cellulase	4810415	Cellulase #	Clostridium thermocellum	0 and 0	Yes	Yes	2445bp (+1)	Add
RcsB	947441	RcsB #	K12 MG1655	0 and 0	Yes	Yes	651bp (+1)	Add
B3023	945805	B3023 #	K12 MG1655	0 and 0	Yes	Yes	483bp (+1)	Add
WaaL	948148	WaaL #	K12 MG1655	0 and 0	Yes	Yes	1260bp (+1)	Add
OtsA	946405	OtsA #	K12 MG1655	0 and 0	Yes	Yes	1425bp (+1)	Add
OtsB	946406	OtsB #	K12 MG1655	0 and 0	Yes	Yes	612bp (+1)	Add
Dam	947893	Dam #	K12 MG1655	0 and 0	Yes	Yes	837bp (+1)	Add
TaqI	6941926	TaqI #	Thermodesulfovibrio yellowstonii DSM 11347	0 and 0	Yes	Yes	777bp (+1)	Add
DpnII	6029545	DpnII #	Strepococcus pneumoniae Hungary19A-6	0 and 0	Yes	Yes	867bp (+1)	Add

Genes and biobrick compatibility

	PAH	Cellulase	rcsB	B3023	waaL	OtsA	OtsB	TaqI	DpnII
EcoRI	No	No	No	No	No	No	No	No	No
SpeI	No	No	No	No	No	No	No	No	No
XbaI	Yes	No	No	No	No	No	No	No	No
PstI	Yes	No	Yes	Yes	No	No	No	No	No
NotI	No	No	No	Yes	No	No	No	No	No
PvuII	Yes (3)	Yes	No	No	No	Yes	No	No	No
XhoI	Yes	No	No	No	No	No	No	No	No
AvrII	No	No	No	No	Yes	No	No	No	No
NheI	No	No	No	No	Yes	No	No	No	No
SapI	No	No	No	No	No	No	No	No	No
Sequence modified?				Yes	Yes