IGEM:IMPERIAL/2009/Encapsulation/Biobricks

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m (Biobricks from the registry)
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<td>Constitutive promoter</td>
<td>Constitutive promoter</td>
<td>BBa_J23103</td>
<td>BBa_J23103</td>
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<td>KP2 2009</td>
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<td>KP1 2009</td>
<td>18I</td>
<td>18I</td>
<td>BBa_J61002</td>
<td>BBa_J61002</td>

Revision as of 17:26, 6 August 2009

Contents

Biobricks

Module 1

Module 1 biobricks

Module 2

Module 2 biobricks

Module 3

Module 3 (provisional) biobricks

Module Timer

Biobricks from the registry

Table 1. Biobricks we need from the registry

Module Part BB # Kit Well # Plasmid Resistance Copy # Link
1 LacIQ promoter BBa_K091111 Shipment: 00352 ID:941 Davidson 2 pSB1A2 A 100-300 LacIQ promoter
1,2,3 RBS BBa_B0034 KP1 2009 2M pSB1A2 A 100-300 RBS
1,2,3 Double terminator BBa_B0015 KP1 2009 23L pSB1AK3 AK 100-300 Double terminator
1,2,3 GFP generator BBa_E0240 KP1 2009 12M pSB1A2 A 100-300 GFP generator
1,2,3 Variable input (testing) BBa_F2620 KP2 2009 6E pSB1A2 A 100-300 Variable input
3 Constitutive promoter BBa_J23114 KP1 2009 20I BBa_J61002 A 15-20 (based on pBR322 ORI) Constitutive promoter
3 Constitutive promoter BBa_J23103 KP1 2009 18I BBa_J61002 A 15-20 (based on pBR322 ORI) Constitutive promoter
3 Promoter (lambda cI regulated) BBa_R0051 KP1 2009 6E pSB1A2 A 100-300 Promoter (lambda cI regulated)
3 cI QPI with high promoter (heat sensitive) BBa K098995 KP3 2009 1E pSB1A2 A 100-300 cI QPI with high promoter
3 pBAD Promoter BBa_I0500 KP1 2009 14N pSB2K3 K Variable – IPTG results in high copy # (++100 per cell) pBAD Promoter
Timer Promoter(luxR & HSL regulated-lux pR) BBa_R0062 KP1 2009 6O pSB1A2 A 100-300 lux pR
Timer Assembly BBa_S03119 KP1 2009 10O pSB1A2 A 100-300 Assembly
Timer LacI-LuxI BBa_K084014 KP2 2009 2B pSB1A2 A 100-300 LacI-LuxI
Timer LacI-LasI BBa_K084007 KP3 2009 12E pSB1AK3 AK 100-300 LacI-LasI
Timer Lac repressible RhlI generator BBa_K084008 KP3 2009 12C pSB1AK3 AK 100-300 Lac repressible RhlI generator


Biobricks

Under construction

Gene NCBI Ref Nucleotide accession# Organism Xbal & SpeI sites Start? Stop? ORF Sequence References
PAH 5053 PAH # Homo Sapiens 1 and 0 Yes Yes 1359bp (+1) Add PAH
Cellulase 4810415 Cellulase # Clostridium thermocellum 0 and 0 Yes Yes 2445bp (+1) Add Add
RcsB 947441 RcsB # K12 MG1655 0 and 0 Yes Yes 651bp (+1) Add Add
B3023 945805 B3023 # K12 MG1655 0 and 0 Yes Yes 483bp (+1) Add Add
WaaL 948148 WaaL # K12 MG1655 0 and 0 Yes Yes 1260bp (+1) Add Add
OtsA 946405 OtsA # K12 MG1655 0 and 0 Yes Yes 1425bp (+1) Add Add
OtsB 946406 OtsB # K12 MG1655 0 and 0 Yes Yes 612bp (+1) Add Add
Dam 947893 Dam # K12 MG1655 0 and 0 Yes Yes 837bp (+1) Add Add
TaqI 6941926 TaqI # Thermodesulfovibrio yellowstonii DSM 11347 0 and 0 Yes Yes 777bp (+1) Add Add
Dpn2 6029545 Dpn2 # Strepococcus pneumoniae Hungary19A-6 0 and 0 Yes Yes 867bp (+1) Add Add


Genes and biobrick compatibility

 

PAH Cellulase rcsB B3023 waaL OtsA OtsB
EcoRI No No No No No No No
SpeI No No No No No No No
XbaI Yes No No No No No No
PstI Yes No Yes Yes No No No
NotI No No No Yes No No No
PvuII Yes (3) Yes No No No Yes No
XhoI Yes No No No No No No
AvrII No No No No Yes No No
NheI No No No No Yes No No
SapI No No No No No No No
Sequence modified?       Yes Yes    
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