IGEM:IMPERIAL/2009/Encapsulation/Biobricks

Biobricks

Module 1

Module 1 biobricks

Module 2

Module 2 biobricks

Module 3

Module 3 (provisional) biobricks

Biobricks from the registry

Table 1. Biobricks we need from the registry

Module	Part	BB #	Kit	Well #	Plasmid	Resistance	Copy #	Link
1,2,3	RBS	BBa_B0034	KP1 2009	2M	pSB1A2	A	100-300	RBS
1,2,3	Double terminator	BBa_B0015	KP1 2009	23L	pSB1AK3	AK	100-300	Double terminator
M1,M2,M3 (Testing)	COMPOSITE PART = GFP generator	BBa_E0240	KP1 2009	12M	pSB1A2	A	100-300	GFP generator
M1,M2,M3 (Testing)	COMPOSITE PART = Variable input (testing)	BBa_F2620	KP2 2009 BB_Barry Box 1	6E	pSB1A2	A	100-300	Variable input
M3, Timer	PROMOTER = Constitutive	BBa_J23114	KP1 2009	20I	BBa_J61002	A	15-20 (based on pBR322 ORI)	Constitutive promoter
M3	PROMOTER = Constitutive	BBa_J23103	KP1 2009	18I	BBa_J61002	A	15-20 (based on pBR322 ORI)	Constitutive promoter
M3	PROMOTER = REPRESSIBLE (lambda cI)	BBa_R0051	KP1 2009	6K	pSB1A2	A	100-300	Promoter (lambda cI regulated)
M3	COMPOSITE PART = HARVARD - cI QPI with high promoter (heat sensitive)	BBa K098995	KP3 2009	1E	pSB1A2	A	100-300	cI QPI with high promoter
M1(M3-Testing)	PROMOTER = INDUCIBLE (pBAD)	BBa_I0500	KP1 2009	14N	pSB2K3	K	Variable – IPTG results in high copy # (++100 per cell)	pBAD Promoter
Timer	PROMOTER = INDUCIBLE (LuxR + HSL)	BBa_R0062	KP1 2009	6O	pSB1A2	A	100-300	lux pR
Timer	PROTEIN GENERATOR = LuxI+RBS	BBa_C0261	KP1 2009	14C	pSB1A2	A	100-300	LuxI Coding + RBS
Timer	PROTEIN GENERATOR = LuxR+RBS	BBa_J37033	KP2 2009	4O	pSB1A2	A	100-300	LuxR Coding + RBS

Biobricks from GeneArt

Table 1. Biobricks we need to synthesise

Gene	Sequence	Link
RBS + DpnII	RBS	BBa_B0034	100-300	RBS

Biobricks

Under construction

Gene	NCBI Ref	Nucleotide accession#	Organism	Xbal & SpeI sites	Start?	Stop?	ORF	Sequence	References
PAH	5053	PAH #	Homo Sapiens	1 and 0	Yes	Yes	1359bp (+1)	Add	PAH
Cellulase	4810415	Cellulase #	Clostridium thermocellum	0 and 0	Yes	Yes	2445bp (+1)	Add	Add
RcsB	947441	RcsB #	K12 MG1655	0 and 0	Yes	Yes	651bp (+1)	Add	Add
B3023	945805	B3023 #	K12 MG1655	0 and 0	Yes	Yes	483bp (+1)	Add	Add
WaaL	948148	WaaL #	K12 MG1655	0 and 0	Yes	Yes	1260bp (+1)	Add	Add
OtsA	946405	OtsA #	K12 MG1655	0 and 0	Yes	Yes	1425bp (+1)	Add	Add
OtsB	946406	OtsB #	K12 MG1655	0 and 0	Yes	Yes	612bp (+1)	Add	Add
Dam	947893	Dam #	K12 MG1655	0 and 0	Yes	Yes	837bp (+1)	Add	Add
TaqI	6941926	TaqI #	Thermodesulfovibrio yellowstonii DSM 11347	0 and 0	Yes	Yes	777bp (+1)	Add	Add
Dpn2	6029545	Dpn2 #	Strepococcus pneumoniae Hungary19A-6	0 and 0	Yes	Yes	867bp (+1)	Add	Add

Genes and biobrick compatibility

	PAH	Cellulase	rcsB	B3023	waaL	OtsA	OtsB
EcoRI	No	No	No	No	No	No	No
SpeI	No	No	No	No	No	No	No
XbaI	Yes	No	No	No	No	No	No
PstI	Yes	No	Yes	Yes	No	No	No
NotI	No	No	No	Yes	No	No	No
PvuII	Yes (3)	Yes	No	No	No	Yes	No
XhoI	Yes	No	No	No	No	No	No
AvrII	No	No	No	No	Yes	No	No
NheI	No	No	No	No	Yes	No	No
SapI	No	No	No	No	No	No	No
Sequence modified?				Yes	Yes