IGEM:IMPERIAL/2009/Encapsulation/Biobricks
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Biobricks
Module 1
Module 2
Module 3
Module 3 (provisional) biobricks
Module Timer
Biobricks from the registry
Table 1. Biobricks we need from the registry
Module | Part | BB # | Kit | Well # | Plasmid | Resistance | Copy # | Link |
1,2,3 | RBS | BBa_B0034 | KP1 2009 | 2M | pSB1A2 | A | 100-300 | RBS |
1,2,3 | Double terminator | BBa_B0015 | KP1 2009 | 23L | pSB1AK3 | AK | 100-300 | Double terminator |
M1,M2,M3 (Testing) | COMPOSITE PART = GFP generator | BBa_E0240 | KP1 2009 | 12M | pSB1A2 | A | 100-300 | GFP generator |
M1,M2,M3 (Testing) | COMPOSITE PART = Variable input (testing) | BBa_F2620 | KP2 2009 BB_Barry Box 1 | 6E | pSB1A2 | A | 100-300 | Variable input |
M3, Timer | PROMOTER = Constitutive | BBa_J23114 | KP1 2009 | 20I | BBa_J61002 | A | 15-20 (based on pBR322 ORI) | Constitutive promoter |
M3 | PROMOTER = Constitutive | BBa_J23103 | KP1 2009 | 18I | BBa_J61002 | A | 15-20 (based on pBR322 ORI) | Constitutive promoter |
M3 | PROMOTER = REPRESSIBLE (lambda cI) | BBa_R0051 | KP1 2009 | 6K | pSB1A2 | A | 100-300 | Promoter (lambda cI regulated) |
M3 | COMPOSITE PART = HARVARD - cI QPI with high promoter (heat sensitive) | BBa K098995 | KP3 2009 | 1E | pSB1A2 | A | 100-300 | cI QPI with high promoter |
M1(M3-Testing) | PROMOTER = INDUCIBLE (pBAD) | BBa_I0500 | KP1 2009 | 14N | pSB2K3 | K | Variable – IPTG results in high copy # (++100 per cell) | pBAD Promoter |
Timer | PROMOTER = INDUCIBLE (LuxR + HSL) | BBa_R0062 | KP1 2009 | 6O | pSB1A2 | A | 100-300 | lux pR |
Timer | PROTEIN GENERATOR = LuxI+RBS | BBa_C0261 | KP1 2009 | 14C | pSB1A2 | A | 100-300 | LuxI Coding + RBS |
Timer | PROTEIN GENERATOR = LuxR+RBS | BBa_J37033 | KP2 2009 | 4O | pSB1A2 | A | 100-300 | LuxR Coding + RBS |
Biobricks from MrGene
Under construction
Table 1. Biobricks we need to synthesise
Biobricks
Under construction
Gene | NCBI Ref | Nucleotide accession# | Organism | Xbal & SpeI sites | Start? | Stop? | ORF | Sequence | References |
PAH | 5053 | PAH # | Homo Sapiens | 1 and 0 | Yes | Yes | 1359bp (+1) | Add | PAH |
Cellulase | 4810415 | Cellulase # | Clostridium thermocellum | 0 and 0 | Yes | Yes | 2445bp (+1) | Add | Add |
RcsB | 947441 | RcsB # | K12 MG1655 | 0 and 0 | Yes | Yes | 651bp (+1) | Add | Add |
B3023 | 945805 | B3023 # | K12 MG1655 | 0 and 0 | Yes | Yes | 483bp (+1) | Add | Add |
WaaL | 948148 | WaaL # | K12 MG1655 | 0 and 0 | Yes | Yes | 1260bp (+1) | Add | Add |
OtsA | 946405 | OtsA # | K12 MG1655 | 0 and 0 | Yes | Yes | 1425bp (+1) | Add | Add |
OtsB | 946406 | OtsB # | K12 MG1655 | 0 and 0 | Yes | Yes | 612bp (+1) | Add | Add |
Dam | 947893 | Dam # | K12 MG1655 | 0 and 0 | Yes | Yes | 837bp (+1) | Add | Add |
TaqI | 6941926 | TaqI # | Thermodesulfovibrio yellowstonii DSM 11347 | 0 and 0 | Yes | Yes | 777bp (+1) | Add | Add |
DpnII | 6029545 | DpnII # | Strepococcus pneumoniae Hungary19A-6 | 0 and 0 | Yes | Yes | 867bp (+1) | Add | Add |
Genes and biobrick compatibility
|
PAH | Cellulase | rcsB | B3023 | waaL | OtsA | OtsB | TaqI | DpnII |
EcoRI | No | No | No | No | No | No | No | No | No |
SpeI | No | No | No | No | No | No | No | No | No |
XbaI | Yes | No | No | No | No | No | No | No | No |
PstI | Yes | No | Yes | Yes | No | No | No | No | No |
NotI | No | No | No | Yes | No | No | No | No | No |
PvuII | Yes (3) | Yes | No | No | No | Yes | No | No | No |
XhoI | Yes | No | No | No | No | No | No | No | No |
AvrII | No | No | No | No | Yes | No | No | No | No |
NheI | No | No | No | No | Yes | No | No | No | No |
SapI | No | No | No | No | No | No | No | No | No |
Sequence modified? | Yes | Yes |