IGEM:IMPERIAL/2009/Feedback & Debriefs/Feedback 31 7

Friday 31st of July 2009

Timer & modeling

• the other way of inducing lac repressor is to use
• IPTG is basically on and off, we might have a number of problems
• leakiness in the lac promoter
• the timer is not directly linked
• reduced tunability
  • rethink the timer?
  • medium defined: put the glucose, put the arabinose, the time delay is directly correlated to the amount of glucose you put in
• how long does it take to make a recombinant protein?
• time delays are massive compared to this
• make the model into a transforming diagram, the new way of communicating the module: in engineering we don't use ODEs, they are hidden in transforms
• what is the Tet repressing well or not?
  • it seems so
• Investigating another solution for M1-M2
  • use an activator, it would be better
  • the current solution wouldn't work (probably)
  • Glucose uptake and energy and protein creation
  • you can buy it: novagen
• taking two existing prts and making a new one out of it, that is positive, still use an activator

Module 3, Killing

• Good test would be using Dam+ and Dam- strains
• Is the lambda Cl going to interfere with lacI
• Why is harvard's 08 biobrick so bad?
  • we should say that at the jamboree
• It has to be a tight promoter

Overview

• Module 1 could take ~5hours
• Module 2 could take ~2hours
• The flow diagrams are good
• we should produce three nice models that we can then link together
• We should probably PCR them all out, much faster
• Using ligation-indipendent cloning can overcome many of the time limitations
• Dam doesn't need synthesis