IGEM:IMPERIAL/2009/Genes

Genes

Presence of restriction enzyme sites

	EcoRI	XbaI	SpeI	PstI
PAH	0	1	0	1
Cellulase	2	0	0	0
RcsB	0	0	0	1
B3023	0	0	0	0
WaaL	0	0	0	0
OtsA	0	0	0	0
OtsB	0	0	0	0
Dam	0	0	0	0
TaqI	0	0	0	0

Primers for Site Directed mutagenesis

NB: The website allowing to make primers for these kits has changed an is now: [1]

PAH XbaI

          t201a	                5'-cctgacccacattgaatccagaccttctcgtttaaag-3'
          t201a_antisense              5'-ctttaaacgagaaggtctggattcaatgtgggtcagg-3'

PAH PstI

          g702t	                5'-gaagacgtttctcaattccttcagacttgcactggtttcc-3'
          g702t_antisense              5'-ggaaaccagtgcaagtctgaaggaattgagaaacgtcttc-3'

Cellulase EcoRI site 1

          c951t	                5'-ggctgtaattatcggagaatttggaaccattgacaagaac-3'
          c951t_antisense	        5'-gttcttgtcaatggttccaaattctccgataattacagcc-3'
         

Cellulase EcoRI site 2

          c1323t	                5'-atattttgcgtggcatagaagaatttccaagtcctagcg-3'
          c1323t_antisense	        5'-cgctaggacttggaaattcttctatgccacgcaaaatat-3'

RcsB

          g369t	                5'-gctctcgccgcgcttcagaaagggaagaaa-3'
          g369t_antisense	        5'-tttcttccctttctgaagcgcggcgagagc-3'