IGEM:IMPERIAL/2009/NYMU timer
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Our timer consists of three parts: starting the timer, counting the time, and "stopping" the timer.
Counting Time
Oscillatory Interface
Our interface only requires that there is a promoter in the oscillatory system such that it shows an oscillatory effect due to the oscillator being in the same cell.
Simplifying the circuit. Oscillatory promoter AND PnhaA = Next
Stopping the timer
We stop the timer by setting a threshold. As the protein produced by the oscillator promoter accumulates and reaches a certain threshold, the output promoter activity would suddenly change