IGEM:IMPERIAL/2009/Primers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
James Field (talk | contribs) |
James Field (talk | contribs) |
||
| Line 13: | Line 13: | ||
ATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTA | ATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTA | ||
AGTCCGGCAGATAAAGACTAA | AGTCCGGCAGATAAAGACTAA | ||
Spec fwd: <b>GCTCTAG</b>ATGAACAATATGAACGTAATTATTGCC 52C | Spec fwd: <b>GCTCTAG</b>ATGAACAATATGAACGTAATTATTGCC 52C | ||
Spec rev: <b>GGACTAGTA</b>TTA GTC TTT ATC TGC CGG ACT TAA GG 56C | Spec rev: <b>GGACTAGTA</b>TTA GTC TTT ATC TGC CGG ACT TAA GG 56C | ||
Revision as of 04:33, 3 August 2009
Primers to be used for PCR
RcsB Sequence:
ATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTG
AGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAA
ACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTA
ATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGA
TTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCC
GAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAA
ATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGG
AAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAA
ATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTA
AGTCCGGCAGATAAAGACTAA
Spec fwd: GCTCTAGATGAACAATATGAACGTAATTATTGCC 52C
Spec rev: GGACTAGTATTA GTC TTT ATC TGC CGG ACT TAA GG 56C
