IGEM:IMPERIAL/2009/Primers: Difference between revisions
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Backward: <b>GGACTAGTA</b>TTAGATACTACGACTAAACGACTC 52C<br> | Backward: <b>GGACTAGTA</b>TTAGATACTACGACTAAACGACTC 52C<br> | ||
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<b>B3023 Sequence:</b><br> | <b>B3023 Sequence:</b><br> | ||
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GCACCGATTCTGTTTCACTACACCAATCTTGCCGAAGGGGTGACAGAGCAGCGACTGGAAACGGAT | GCACCGATTCTGTTTCACTACACCAATCTTGCCGAAGGGGTGACAGAGCAGCGACTGGAAACGGAT | ||
GTTTATGTGCCGTTGGCGTGA | GTTTATGTGCCGTTGGCGTGA | ||
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Spec fwd: <b>GCTCTAG</b>ATGACAAACCTGACACTGGATG 53C<br> | |||
Spec rev: <b>GGACTAGTA</b>TCACGCCAACGGCACATAAAC 54C<br> | |||
<br> | |||
<b>WaaL Sequence:</b><br> | <b>WaaL Sequence:</b><br> | ||
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TATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAA | TATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAA | ||
<br> | |||
Spec fwd: <b>GCTCTAG</b>ATGCTAACATCCTTTAAACTTCATTC 52C | Spec fwd: <b>GCTCTAG</b>ATGCTAACATCCTTTAAACTTCATTC 52C<br> | ||
Spec rev: <b>GGACTAGTA</b>TTAATTAATTGTATTGTTACGATTATTAATG 50C | Spec rev: <b>GGACTAGTA</b>TTAATTAATTGTATTGTTACGATTATTAATG 50C | ||
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Revision as of 04:49, 3 August 2009
Primers to be used for PCR
RcsB Sequence:
ATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTG
AGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAA
ACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTA
ATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGA
TTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCC
GAAAGCTCTCGCCGCGCTGCAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAA
ATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGG
AAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAA
ATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTA
AGTCCGGCAGATAAAGACTAA
Spec fwd: GCTCTAGATGAACAATATGAACGTAATTATTGCC 52C
Spec rev: GGACTAGTATTA GTC TTT ATC TGC CGG ACT TAA GG 56C
OtsB Sequence:
ATGGTGGAGCTTGACGCACTGGCAAAACCTTATCGCTTCCCGTTAGCGGGCGTGCATGGGGC
GGAGCGCCGTGACATCAATGGTAAAACACATATCGTTCATCTGCCGGATGCGATTGCGCGTGAT
ATTAGCGTGCAACTGCATACAGTCATCGCTCAGTATCCCGGCGCGGAGCTGGAGGCGAAAGGGA
TGGCTTTTGCGCTGCATTATCGTCAGGCTCCGCAGCATGAAGACGCATTAATGACATTAGCGCA
ACGTATTACTCAGATCTGGCCACAAATGGCGTTACAGCAGGGAAAGTGTGTTGTCGAGATCAAA
CCGAGAGGTACCAGTAAAGGTGAGGCAATTGCAGCTTTTATGCAGGAAGCTCCCTTTATCGGGC
GAACGCCCGTATTTCTGGGCGATGATTTAACCGATGAATCTGGCTTCGCAGTCGTTAACCGACT
GGGCGGAATGTCAGTAAAAATTGGCACAGGTGCAACTCAGGCATCATGGCGACTGGCGGGTGTG
CCGGATGTCTGGAGCTGGCTTGAAATGATAACCACCGCATTACAACAAAAAAGAGAAAATAACA
GGAGTGATGACTATGAGTCGTTTAGTCGTAGTATCTAA
Forward: GCTCTAGATGGTGGAGCTTGACGCAC 53C
Backward: GGACTAGTATTAGATACTACGACTAAACGACTC 52C
B3023 Sequence:
ATGACAAACCTGACACTGGATGTAAACATTATCGATTTCCCATCAATACCTGTGGCGATGTTGCCG
CACCGCTGTAGCCCTGAATTGCTCAACTACAGCGTGGCGAAATTTATCATGTGGCGTAAAGAGACG
GGGCTTTCTCCTGTTAACCAAAGCCAGACTTTTGGCGTCGCCTGGGACGACCCTGCCACCACCGCA
CCGGAAGCGTTTCGCTTTGATATCTGCGGCAGCGTTAGCGAACCGATTCCCGATAATCGTTATGGT
GTGAGCAATGGTGAACTTACCGGTGGACGTTATGCCGTGGCCCGCCACGTTGGCGAGCTGGACGAT
ATTTCACACACGGTATGGGGCATCATTCGCCACTGGCTGCCTGCAAGCGGCGAGAAAATGCGTAAA
GCACCGATTCTGTTTCACTACACCAATCTTGCCGAAGGGGTGACAGAGCAGCGACTGGAAACGGAT
GTTTATGTGCCGTTGGCGTGA
Spec fwd: GCTCTAGATGACAAACCTGACACTGGATG 53C
Spec rev: GGACTAGTATCACGCCAACGGCACATAAAC 54C
WaaL Sequence:
ATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAG ATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATA AAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATT ATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTA TTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAA ATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGA GTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCAT TCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTA ATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACTAAGAAAAATCATCCTTTTTTATTTTTATTAA ATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTAT AATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTT CTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATG AAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGC AATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGC GCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGCATTGGAGTTTTCTAACG TACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACT TTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTTTGTTGATATTAACG CTTGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTA TATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAA
Spec fwd: GCTCTAGATGCTAACATCCTTTAAACTTCATTC 52C
Spec rev: GGACTAGTATTAATTAATTGTATTGTTACGATTATTAATG 50C
