IGEM:IMPERIAL/2009/Sun cream: Difference between revisions
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Tocochromanols are only synthesised in photosynthetic organisms; they are powerful antioxidants that protect the organism against reactive oxygen species (ROS). There has been successful reproduction of the biosynthetic pathway in <i>E.coli</i> through characterisation of the genes involved by knock-out and mutant studies. <i>E.coli</i> would be the chassis of choice. | Tocochromanols are only synthesised in photosynthetic organisms; they are powerful antioxidants that protect the organism against reactive oxygen species (ROS). There has been successful reproduction of the biosynthetic pathway in <i>E.coli</i> through characterisation of the genes involved by knock-out and mutant studies. <i>E.coli</i> would be the chassis of choice. | ||
<i>Table from Ref 1:</i> | <i>Table from Ref <cite>#1</cite>:</i> | ||
<b><i>Table 1. Strains and plasmids used in this work.</i></b> | <b><i>Table 1. Strains and plasmids used in this work.</i></b> | ||
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</table> | </table> | ||
<br> | <br> | ||
<center>[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany). | <center><i>[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany). | ||
</center> | </i></center> | ||
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==References== | ==References== | ||
<biblio> | |||
#1[http://interscience.wiley.com/cgi-bin/fulltext/121413867/PDFSTART 1] | |||
</biblio> | |||
Latest revision as of 19:06, 19 July 2009
Sun Cream
Description
Development phases
Phase 1 - Compounds/threshold/detection
Tocochromanols are only synthesised in photosynthetic organisms; they are powerful antioxidants that protect the organism against reactive oxygen species (ROS). There has been successful reproduction of the biosynthetic pathway in E.coli through characterisation of the genes involved by knock-out and mutant studies. E.coli would be the chassis of choice.
Table from Ref [1]:
Table 1. Strains and plasmids used in this work.
| Strain or plasmid | Relevant properties or genotype | Source Strains |
| E. coli DH5 | F-, 80d, lacZ M15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169 | [a] |
| E. coli M15 [pREP4] | F-, lacZ M15, thi-1, lac-, mtl-, recA+ , KmR | [a] |
| Pseudomonas putida KT2440 | type strain | [b] |
| Synechocystis sp. PCC 6803 | type strain | [c] |
| pCAR16 | -carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR | [27] |
| pJF119EH | cloning vector, tac-promoter, IPTG inducible, AmpR | [25] |
| pJF119 N | cloning vector, RBS, tac-promoter, IPTG inducible, AmpR | this study |
| pAW229 | cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR | [26] |
| pQE31-VTE1 | tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR | [18a] |
| pCAS2JF | 1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH | this study |
| pCAS8 | 1.1 kb NdeI/BamHI PCR fragment ggh in pJF119 N | this study |
| pCAS11 | 0.9 kb BglII/BamHI PCR fragment crtE in pCAS8 | this study |
| pCAS12 | 1.0 kb BglII/BamHI PCR fragment hpt in pCAS11 | this study |
| pCAS15 | 1.2 kb NdeI/BamHI PCR fragment hpd in pAW229 | this study |
| pCAS18 | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS11 | this study |
| pCAS19 | 1.0 kb NdeI/BamHI PCR fragment hpt in pAW229 | this study |
| pCAS23 | 1.0 kb NdeI/BamHI PCR fragment hpt in pJF119 N | this study |
| pCAS24 | 0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23 | this study |
| pCAS27 | 1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229 | this study |
| pCAS29 | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS18 | this study |
| pCAS30 | 0.9 kb NdeI/BamHI PCR fragment crtE in pJF119 N | this study |
| pCAS37 | 1.2 kb BglII/BamHI PCR fragment hpd in pCAS24 | this study |
| pCAS47 | 1.3 kb BglII PCR fragment vte1 in pCAS29 | this study |
