IGEM:IMPERIAL/2009/Sun cream

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==References==
==References==
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Revision as of 20:57, 19 July 2009

Contents

Sun Cream

Description

Development phases

Phase 1 - Compounds/threshold/detection

Phase 2 - Encapsulation

Phase 3 - Killing strategy/gene deletion

Phase 4 - Storage

Phase 5 - Delivery

References

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid Relevant properties or genotype Source

Strains
E. coli DH5 F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169 [a]
E. coli M15 [pREP4] F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR [a]
Pseudomonas putida KT2440 type strain [b]
Synechocystis sp. PCC 6803 type strain [c]
Plasmids
pCAR16 -carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR [27]
pJF119EH cloning vector, tac-promoter, IPTG inducible, AmpR [25]
pJF119N cloning vector, RBS, tac-promoter, IPTG inducible, AmpR this study
pAW229 cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR [26]
pQE31-VTE1 tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR [18a]
pCAS2JF 1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH this study
pCAS8 1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N this study
pCAS11 0.9 kb BglII/BamHI PCR fragment crtE in pCAS8 this study
pCAS12 1.0 kb BglII/BamHI PCR fragment hpt in pCAS11 this study
pCAS15 1.2 kb NdeI/BamHI PCR fragment hpd in pAW229 this study
pCAS18 1.2 kb BglII/BamHI PCR fragment hpd in pCAS11 this study
pCAS19 1.0 kb NdeI/BamHI PCR fragment hpt in pAW229 this study
pCAS23 1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N this study
pCAS24 0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23 this study
pCAS27 1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229 this study
pCAS29 1.2 kb BglII/BamHI PCR fragment hpd in pCAS18 this study
pCAS30 0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N this study
pCAS37 1.2 kb BglII/BamHI PCR fragment hpd in pCAS24 this study
pCAS47 1.3 kb BglII PCR fragment vte1 in pCAS29 this study

[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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