IGEM:IMPERIAL/Protocols/20ul digestion: Difference between revisions

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== 20ul digestion ==
 
5~8ug  DNA (inserts, if vector, max of 0.5ug)
2ul  10x buffer
2ul  Enzyme – add last (must be kept cold at all times)
(should be less than 10% of the total volume  otherwise
glycerol will inhibit the enzyme activity)
Make it up to 20ul with MiliQ
1hr at 37oC.

Latest revision as of 05:18, 28 October 2006