IGEM:IMPERIAL/Protocols/J37015-LuxI: Difference between revisions
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The motivation behind this protocol is to directly assessing the concentration of LuxI in the prey cell using a downstream GFP gene as a measurement. This is done as an alternative to measuring AHL indirectly using the T9002 assay. | The motivation behind this protocol is to directly assessing the concentration of LuxI in the prey cell using a downstream GFP gene as a measurement. This is done as an alternative to measuring AHL indirectly using the T9002 assay. | ||
The concentrations of LuxI and AHL have so far been assumed to be equal. This protocol will allow us to compare the behaviour of the two chemicals, and if indeed, our assumption proves to be correct, the measurement of LuxI protocol can completely replace the less accurate indirect AHL measurement protocol. | The concentrations of LuxI and AHL have so far been assumed to be equal. This protocol will allow us to compare the behaviour of the two chemicals, and if indeed, our assumption proves to be correct, the measurement of LuxI protocol can completely replace the less accurate indirect AHL measurement [[IGEM:IMPERIAL/Protocols/J37015|protocol]]. | ||
===Equipment & Materials=== | ===Equipment & Materials=== | ||
Revision as of 02:57, 23 August 2006
Part J37015 Alternative Protocol: Measuring LuxI
Motivation
The motivation behind this protocol is to directly assessing the concentration of LuxI in the prey cell using a downstream GFP gene as a measurement. This is done as an alternative to measuring AHL indirectly using the T9002 assay. The concentrations of LuxI and AHL have so far been assumed to be equal. This protocol will allow us to compare the behaviour of the two chemicals, and if indeed, our assumption proves to be correct, the measurement of LuxI protocol can completely replace the less accurate indirect AHL measurement protocol.
Equipment & Materials
- Equipment
- Wallac Victor 3 Multi-Well Fluorimeter
- Ependorf Tubes
- Gilson Pippettes
- 37[[:Category:{{{1}}}|{{{1}}}]] Shaker
- Microfuge
- Materials
- E.coli Growth Medium w/Ampicilin
- E.coli Culture Containing T9002
- E.coli Culture Containing J37015
- GFP Standard Solution
