IGEM:Imperial/2007/Wet Lab/results/CBD2.3

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==Aims==
==Aims==
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To determine the optimum concentration of [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 '''pTet-GFPmut3b'''] ''in vitro''.
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To determine how the system ([http://parts.mit.edu/registry/index.php/Part:BBa_I13522 '''pTet-GFPmut3b''']) responds to a step increase or a step decrease in temperature, compared to a constant temperture, ''in vitro''.
== Materials and Methods ==
== Materials and Methods ==

Revision as of 17:23, 24 October 2007

In vitro Testing of pTet-GFPmut3b Construct for constant and step temperatures

Aims

To determine how the system (pTet-GFPmut3b) responds to a step increase or a step decrease in temperature, compared to a constant temperture, in vitro.

Materials and Methods

Link to the Protocols page

Results

Average fluorescence over time for two samples at constant temperatures of 4&degC and 20°C and two with a temperature step ( 4-20°C and 20-4°C)


Controls:

  • Negative control - empty vector (pTet promoter) with the cell extract

Discussion



Conclusion

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