IGEM:Imperial/2007/Wet Lab/results/CBD2.3

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== Results ==
== Results ==
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[[Image:IC 2007 Temp step.PNG|Average fluorescence over time for two samples at constant temperatures of 4&degC and 20°C and two with a temperature step ( 4-20°C and 20-4°C) ]]
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| width="600px"|<br>[[Image:IC 2007 Temp step.PNG|Average fluorescence over time for two samples at constant temperatures of 4&degC and 20&deg;C and two with a temperature step ( 4-20&deg;C and 20-4&deg;C)|thumb|600px|Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration ''in vitro'' - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b ''in vitro'' using our calibration curve.]]  
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Revision as of 16:27, 24 October 2007

In vitro Testing of pTet-GFPmut3b Construct for constant and step temperatures

Aims

To determine how the system (pTet-GFPmut3b) responds to a step increase or a step decrease in temperature, compared to a constant temperture, in vitro.

Materials and Methods

Link to the Protocols page

Results


Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration in vitro - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b in vitro using our calibration curve.
Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration in vitro - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b in vitro using our calibration curve.



Controls:

  • Negative control - empty vector (pTet promoter) with the cell extract

Discussion



Conclusion

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