IGEM:Imperial/2007/Wet Lab/results/CBD2.3: Difference between revisions
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== Results == | == Results == | ||
[[Image:IC 2007 Temp step.PNG|Average fluorescence over time for two samples at constant temperatures of 4°C and 20°C and two with a temperature step ( 4-20°C and 20-4°C) ]] | {|align="center" | ||
| width="600px"|<br>[[Image:IC 2007 Temp step.PNG|Average fluorescence over time for two samples at constant temperatures of 4°C and 20°C and two with a temperature step ( 4-20°C and 20-4°C)|thumb|600px|Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration ''in vitro'' - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b ''in vitro'' using our calibration curve.]] | |||
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Revision as of 14:27, 24 October 2007
In vitro Testing of pTet-GFPmut3b Construct for constant and step temperatures
Aims
To determine how the system (pTet-GFPmut3b) responds to a step increase or a step decrease in temperature, compared to a constant temperture, in vitro.
Materials and Methods
Link to the Protocols page
Results
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Controls:
- Negative control - empty vector (pTet promoter) with the cell extract
Discussion
