IGEM:Imperial/2007/Wet Lab/results/CBD2.3

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In vitro Testing of pTet-GFPmut3b Construct for constant and step temperatures

Aims

To determine the optimum concentration of pTet-GFPmut3b in vitro.

Materials and Methods

Link to the Protocols page

Results

Average fluorescence over time for two samples at constant temperatures of 4&degC and 20°C and two with a temperature step ( 4-20°C and 20-4°C)


Controls:

  • Negative control - empty vector (pTet promoter) with the cell extract

Discussion



Conclusion