IGEM:Imperial/2007/Wet Lab/results/CBD4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Maira Tariq (talk | contribs) m (→Results) |
Maira Tariq (talk | contribs) m (→Results) |
||
Line 12: | Line 12: | ||
==Results== | ==Results== | ||
{|align="center" | {|align="center" | ||
| width="600px"|<br>[[Image:IC 2007 DNA conc CBD.PNG|thumb|600px|Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration ''in vitro'' - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b ''in vitro'' using our calibration curve | | width="600px"|<br>[[Image:IC 2007 DNA conc CBD.PNG|thumb|600px|Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration ''in vitro'' - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b ''in vitro'' using our calibration curve.]] | ||
|} | |} | ||
Revision as of 07:38, 24 October 2007
Testing DNA concentration of pTet-GFPmut3b In vitro
Aims
To determine if the optimum concentration of pTet-GFPmut3b in vitro.
Materials and Methods
Refer to protocols page.
Tested on (Insert link)
Results
Controls:
- Negative Control- Nuclease Free Water was added instead of DNA
Constants:
- Temperature - 37°C
- Total Volume - 60µl
Raw Data