IGEM:Imperial/2007/Wet Lab/results/CBD4: Difference between revisions

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m (New page: = Testing DNA concentration of pTet-GFPmut3b ''In vitro''= __NOTOC__ ==Aims== To determine if the optimum concentration of [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 '''pTet-...)
 
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__NOTOC__
__NOTOC__
==Aims==
==Aims==
To determine if the optimum concentration of [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 '''pTet-GFPmut3b'] ''in vitro''.
To determine if the optimum concentration of [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 '''pTet-GFPmut3b'] ''in vitro''.


==Materials and Methods==
==Materials and Methods==

Revision as of 07:46, 23 October 2007

Testing DNA concentration of pTet-GFPmut3b In vitro

Aims

To determine if the optimum concentration of pTet-GFPmut3b' in vitro.

Materials and Methods

Refer to protocols page.
Tested on (Insert link)

Results


File:D.PNG
Fig.1.1:DNA Concentration in vitro - The DNA concentration of pTet-GFPmut3b in vitro. The fluorescence was measured and converted into molecules of GFPmut3b in vitro using our calibration curve.

File:.PNG
Fig.1.2:DNA Concentration in vitro after 360 minutes - This graph shows the molecules of GFPmut3b produced after 360 minutes at varying DNA concentrations.


Controls:

  • Negative Control- Nuclease Free Water was added instead of DNA

Constants:

  • Temperature - 37°C
  • Total Volume - 60µl

Raw Data

Discussion

Conclusion

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