IGEM:Imperial/2007/Wet Lab/results/CBD4

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==Results==
==Results==
{|align="center"
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| width="600px"|<br>[[Image:IC 2007 DNA conc CBD.PNG|thumb|600px|Fig.1.1:DNA Concentration ''in vitro'' - The DNA concentration of pTet-GFPmut3b ''in vitro''.]]  
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| width="600px"|<br>[[Image:IC 2007 DNA conc CBD.PNG|thumb|600px|Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration ''in vitro'' - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b ''in vitro'' using our calibration curve.]] .]]  
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{|align="center"
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| width="600px"|<br>[[Image:IC 2007 DNA conc @360CBD.PNG|thumb|600px|Fig.1.2:DNA Concentration ''in vitro'' after 360 minutes - This graph shows the fluorescence produced after 360 minutes at varying DNA concentrations.]]  
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| width="600px"|<br>[[Image:IC 2007 DNA conc @360CBD.PNG|thumb|600px|Fig.1.2:Molecules of GFPmut3b synthesised for each DNA Concentration ''in vitro'', after 360 minutes]]  
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Revision as of 09:37, 24 October 2007

Testing DNA concentration of pTet-GFPmut3b In vitro

Aims

To determine if the optimum concentration of pTet-GFPmut3b in vitro.

Materials and Methods

Refer to protocols page.
Tested on (Insert link)

Results


Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration in vitro - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b in vitro using our calibration curve.
Fig.1.1:Molecules of GFPmut3b synthesised over time, for each DNA Concentration in vitro - The fluorescence was measured over time for each experiment and converted into molecules of GFPmut3b in vitro using our calibration curve.
.]]

Fig.1.2:Molecules of GFPmut3b synthesised for each DNA Concentration in vitro, after 360 minutes
Fig.1.2:Molecules of GFPmut3b synthesised for each DNA Concentration in vitro, after 360 minutes


Controls:

  • Negative Control- Nuclease Free Water was added instead of DNA

Constants:

  • Temperature - 37°C
  • Total Volume - 60µl

Raw Data

Discussion

Conclusion

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