IGEM:Imperial/2007/Wet Lab/results/CBD4

Testing DNA concentration of pTet-GFPmut3b In vitro

Aims

To determine if the optimum concentration of pTet-GFPmut3b' in vitro.

Materials and Methods

Refer to protocols page.
Tested on (Insert link)

Results

Fig.1.1:DNA Concentration in vitro - The DNA concentration of pTet-GFPmut3b in vitro. The fluorescence was measured and converted into molecules of GFPmut3b in vitro using our calibration curve.

File:.PNG Fig.1.2:DNA Concentration in vitro after 360 minutes - This graph shows the molecules of GFPmut3b produced after 360 minutes at varying DNA concentrations.

Controls:

• Negative Control- Nuclease Free Water was added instead of DNA

Constants:

• Temperature - 37°C
• Total Volume - 60µl

Raw Data

• Raw Data excel file