IGEM:Imperial/2007/Wet Lab/results/CBD4
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Testing DNA concentration of pTet-GFPmut3b In vitro
Aims
To determine if the optimum concentration of pTet-GFPmut3b' in vitro.
Materials and Methods
Refer to protocols page.
Tested on (Insert link)
Results
[[|thumb|600px|Fig.1.1:DNA Concentration in vitro - The DNA concentration of pTet-GFPmut3b in vitro. The fluorescence was measured and converted into molecules of GFPmut3b in vitro using our calibration curve.]] |
Controls:
- Negative Control- Nuclease Free Water was added instead of DNA
Constants:
- Temperature - 37°C
- Total Volume - 60µl
Raw Data