IGEM:Imperial/2010/Diary/Week7

This was a really busy week in the lab. All three teams made really good progress, and we all really appreciated finally being able to do something practical for the project.

The XylE team miniprepped plasmids containing the XylE gene, which was used for assays for C2,3O activity. They also used 3A assembly to join the J23101 (promoter), XylE and pSB1C3 together. At the same time, they started the construction of the GFP-XylE fusion protein.

The surface protein team spent the week cloning the double terminator (B0014) part into pSB1C3, which will be needed at various points in the assembly strategy.

On Tuesday we were lucky enough to meet Jay Keasling, who was really inspiring and very positive about our project. We were plied with drinks by our advisors (which we had no objection to) and then ended the night with a few drinks at the union.

Here's a photo of the team with Jay Keasling:

On Thursday, the modellers had a meeting with Dr Bultelle regarding the output amplification model and the protein display model.

Kirill bought a strawberry cheesecake for Cake Friday.