IGEM:Imperial/2010/Diary/Week7

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This was a really busy week in the lab. All three teams made really good progress, and we all really appreciated finally being able to do something practical for the project.

The XylE team miniprepped plasmids containing the XylE gene, which was used for assays for C2,3O activity. They also used 3A assembly to join the J23101 (promoter), XylE and pSB1C3 together. At the same time, they started the construction of the GFP-XylE fusion protein.

The surface protein team spent the week cloning the double terminator (B0014) part into pSB1C3, which will be needed at various points in the assembly strategy.

On Thursday, the modellers had a meeting with Dr Bultelle regarding the output amplification model and the protein display model. Piotr: a few details please!

Kirill bought a strawberry cheesecake for Cake Friday.

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