IGEM:Imperial/2010/Diary/Week8
This week, the XylE Team continued to work on the GFP-XylE fusion protein. Wolf joined the team to help with the workload, and the catechol assays were started. On Tuesday we were lucky enough to meet Jay Keasling, who was really inspiring and very positive about our project. We were plied with drinks by our advisors (which we had no objection to) and then ended the night with a few drinks at the union. The Surface Protein Team did a PCR to obtain the LytC cell wall binding domain (CWBD) from the B. subtilis genome for ligation into pSB1C3 and subsequently transformation into E. coli. They also started cloning pVEG.
On Thursday night, we all went to Nick’s house for pizza and a few drinks. Kirill graced Earl’s Court with a naked run and Piotr showed off his awesome dancing.
For Cake Friday, Florian made a very impressive homemade cheesecake. Clearly his cooking skills are as good as his lab skills!