IGEM:Imperial/2010/Fast Response module: Difference between revisions
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| [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless | | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless | ||
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| Activation (phosphorylation) | | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]] | ||
| Enz-in-pathway | | Enz-in-pathway | ||
Revision as of 09:06, 15 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) |
|---|---|
| transcr sigma 54 | 2 colourless |
| Activation (phosphorylation) | Enz-in-pathway
|
Target proteases to use: Piotr, Nick, Kyasha
|
Protein scaffold: Maddie |
| 2C DNA binding prot release |
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |
