IGEM:Imperial/2010/Fast Response module/Activation phosphorylation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Quorum Sensing in B.substilis== The idea is to fuse our split protease on the components of the quorum sensing system of B.substilis. The signal peptide is the product of ComX gene. The...)
m (Quorum Sensing in B.substilis)
Line 2: Line 2:
The idea is to fuse our split protease on the components of the quorum sensing system of B.substilis. The signal peptide is the product of ComX gene. The sensor is the transmembrane receptor ComP which autophosphorylates(the first half of the split protease will be attached on ComP). ComA is the relay compoment which gets phosphorylated by ComP (the second part of the split protease will be attached on the ComA). When ComP and Coma associate that would produce the active protease.
The idea is to fuse our split protease on the components of the quorum sensing system of B.substilis. The signal peptide is the product of ComX gene. The sensor is the transmembrane receptor ComP which autophosphorylates(the first half of the split protease will be attached on ComP). ComA is the relay compoment which gets phosphorylated by ComP (the second part of the split protease will be attached on the ComA). When ComP and Coma associate that would produce the active protease.
 +
 +
*'''Chris D Hirst 12:12, 15 July 2010 (EDT)''': What causes expression of ComX under normal circumstances in the cell?
==References==
==References==
A useful review on quorum sensing. look at the middle of the article for gram positive bacteria
A useful review on quorum sensing. look at the middle of the article for gram positive bacteria
[http://arjournals.annualreviews.org/doi/full/10.1146/annurev.micro.55.1.165?amp;searchHistoryKey=%24{searchHistoryKey}| Quorum sensing]]
[http://arjournals.annualreviews.org/doi/full/10.1146/annurev.micro.55.1.165?amp;searchHistoryKey=%24{searchHistoryKey}| Quorum sensing]]

Revision as of 12:12, 15 July 2010

Quorum Sensing in B.substilis

The idea is to fuse our split protease on the components of the quorum sensing system of B.substilis. The signal peptide is the product of ComX gene. The sensor is the transmembrane receptor ComP which autophosphorylates(the first half of the split protease will be attached on ComP). ComA is the relay compoment which gets phosphorylated by ComP (the second part of the split protease will be attached on the ComA). When ComP and Coma associate that would produce the active protease.

  • Chris D Hirst 12:12, 15 July 2010 (EDT): What causes expression of ComX under normal circumstances in the cell?

References

A useful review on quorum sensing. look at the middle of the article for gram positive bacteria Quorum sensing]

Personal tools