IGEM:Imperial/2010/Michaelis Menten: Difference between revisions
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==Improved Model (18/07/2010)== | ==Improved Model which accounts for enzyme (Protease reaction) (18/07/2010)== | ||
[[Image:Model_output_020.jpg.jpg|450px|thumb|center|alt=A|Model improved to account for the enzymes (protease action) ]] | [[Image:Model_output_020.jpg.jpg|450px|thumb|center|alt=A|Model improved to account for the enzymes (protease action) ]] | ||
Revision as of 03:35, 2 September 2010
Model based on Michaelis Menten Kinetics (Weeks 4 and 5)
Motivation
We came up with a simple concept of output amplification, which is enhanced by using enzymes. It is beneficial for us to model the behaviour of our design so that we will be able to answer the following questions.
- How beneficial is the use of amplification? (Compare speed of response of transcription (and translation) with 1- or 2-step amplification)
- How many amplification steps are beneficial to have? Will further adding of amplification steps introduce too many time delays?
- Is it better to use TEV all or HIV1?
Modelling should allows us to make a decision on which design is the most efficient one.
First Model
HIV1
Equations
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Parameters
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TEV
Equations
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Parameters
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Improved Model which accounts for enzyme (Protease reaction) (18/07/2010)
Implementation in Matlab
The Matlab code for the different stages of amplification and diagrams can be found here.
Kinetic constants
Quality | GFP | TEV | split TEV | split GFP |
---|---|---|---|---|
Km and Kcat | Doesn't apply | TEV constants (Km and kcat) | 40% of whole TEV | Doesn't apply |
half-life or degradation rate | Half-life of GFP in Bacillus = 1.5 hours - ref. Chris | ? | ? | Half-life shorter than GFP |
production rate in B.sub | ? | ? | ? | ? |
Conclusion
We couldn't obtain all the necessary constants. Hence, we decided to make educated guesses about possible relative values between the constants as well as varying them and observing the change in output.
As the result, we concluded that the amplification happens at each amplification level proposed. It's magnitude varies depending on the constants. There doesn’t seem to be much difference in substitution of TEV with HIV1.