IGEM:Imperial/2010/Michaelis Menten: Difference between revisions
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'''Equations''' | '''Equations''' | ||
*m | |||
*p_h | |||
* | *<math>\dot{m}=k_o - d_om</math> | ||
*p_g | |||
*<math>\dot{p_h} = k_hm - d_hp_h</math> | |||
*<math>\dot{p_t} = k_tp_h - d_tp_t</math> | |||
*<math>\dot{p_g} = k_gp_t - d_gp_g</math> | |||
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'''Parameters''' | '''Parameters''' | ||
* | *k_o...transcription rate of HIV1 | ||
* | *d_o...degradation rate ogf mRNA coding for HIV1 | ||
*k_h...translation rate of HIV1 | *k_h...translation rate of HIV1 | ||
*d_h...degradation rate of HIV1 | *d_h...degradation rate of HIV1 | ||
* | *k_t...production rate of TEV by HIV1 | ||
* | *d_t...degradation rate of TEV | ||
*k_g...production rate of GFP by TEB | *k_g...production rate of GFP by TEB | ||
*d_g...degradation rate of GFP | *d_g...degradation rate of GFP |
Revision as of 07:07, 2 September 2010
Model based on Michaelis Menten Kinetics (Weeks 4 and 5)
Motivation
We came up with a simple concept of output amplification, which is enhanced by using enzymes. It is beneficial for us to model the behaviour of our design so that we will be able to answer the following questions.
- How beneficial is the use of amplification? (Compare speed of response of transcription (and translation) with 1- or 2-step amplification)
- How many amplification steps are beneficial to have? Will further adding of amplification steps introduce too many time delays?
- Is it better to use TEV all or HIV1?
Modelling should allows us to make a decision on which design is the most efficient one.
First Model
HIV1
Equations
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Parameters
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TEV
Equations
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Parameters
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Improved Model which accounts for enzyme reactions (28/07/2010)
TEV
Equations
p_t' = s_t - d_t * p_t; where s_t = (k_t * k_to)/d_to
p_st' = s_st - d_st * p_st
p_sg' = s_sg - d_sg * p_sg
p_ts' = (V_max,t * [p_st])/(K_m,ts + [p_st]) - d_ts * p_ts
p_g' = (V_max,tg * [p_sg])/(K_m,tg + [p_sg]) + (V_max,tsg * [p_sg])/(K_m,tsg + [p_sg]) - d_g * p_g |
Implementation in Matlab
The Matlab code for the different stages of amplification and diagrams can be found here.
Kinetic constants
GFP | TEV | split TEV | split GFP | |
---|---|---|---|---|
Km and Kcat | - | Km = 0.061; Kcat = 0.16; [1] | 40% of value for TEV | - |
Half-life or degradation rate | Half-life in B.sub approximately 1.5 hours | ? | ? | Half-life shorter than GFP |
Production rate in B.sub | ? | ? | ? | ? |
Conclusion
We were not able to obtain all the necessary constants. Hence, we decided to make educated guesses about possible relative values between the constants as well as varying them and observing the change in output.
As the result, we concluded that the amplification happens at each amplification level proposed. The magnitude of amplification varies depending on the constants. There is not much difference between using TEV or HIV1.
References
- Kapust R. et al (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Engineering. [Online] 14(12), 993-1000. Available from: http://peds.oxfordjournals.org/cgi/reprint/14/12/993 [Accessed 28th July 2010]