IGEM:Imperial/2010/Output module: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Added Bilins link) |
No edit summary |
||
| Line 34: | Line 34: | ||
#2 pmid=17072307 | #2 pmid=17072307 | ||
</biblio> | </biblio> | ||
==GFP as output== | |||
[http://www.nature.com/nmeth/journal/v3/n10/suppinfo/nmeth932_S1.html In vivo and in vitro protein solubility assays using split GFP] | |||
Revision as of 04:11, 28 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Bilins |
| Activation (phosphorylation) | Enz-in-pathway
|
Anthocyanins
|
Target proteases to use
|
Protein scaffold | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |
GFP as output
In vivo and in vitro protein solubility assays using split GFP
