IGEM:Imperial/2010/Strategy: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 23: | Line 23: | ||
[[Image:PrimersPic1.bmp|thumb|right|600px|x400px| '''Additional primers - work in progress''']] | [[Image:PrimersPic1.bmp|thumb|right|600px|x400px| '''Additional primers - work in progress''']] | ||
* 14 other primers will be required. Design is in progress (pic of word | * 14 other primers will be required. Design is in progress (pic of word tabl)- have been ordered (OK) | ||
* Experimental design for XylE e.coli testing! | * Experimental design for XylE e.coli testing! (OK) | ||
* Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly! | * Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly! | ||
* Strategies for testing of expression etc have to be developed and structured. (OK) | |||
* Strategies for testing of expression etc have to be developed and structured. | |||
* List of parts for submission has to be assembled. | * List of parts for submission has to be assembled. | ||
* ComE promoter system - recap on mode of function. | * ComE promoter system - recap on mode of function. | ||
[[Image:Primers1.1.docx]] | [[Image:Primers1.1.docx]] | ||
Revision as of 03:44, 10 September 2010
Strategy
Please refer to the assembly strategy for details.
Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.
- XylE team (Maddie and Nicolas)
- Vectors team (Kyasha and Kirill)
- Surface Protein team (Harriet and Florian)
Key updates:
- XylE characterization: During the next week (week 9) we will start characterization of XylE. Purification of XylE-His tagged from Jeremy's cultures will be a first step in direction of in-vitro assays. Upon assembly of the GFP-XylE fusion protein this week, protein purification will provide us with both XylE and GFP-XylE for in vitro testing. Important kinetic data will hopefully be obtained during the next 7 days.
Assembly strategy
Wolfs checklist
- 14 other primers will be required. Design is in progress (pic of word tabl)- have been ordered (OK)
- Experimental design for XylE e.coli testing! (OK)
- Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
- Strategies for testing of expression etc have to be developed and structured. (OK)
- List of parts for submission has to be assembled.
- ComE promoter system - recap on mode of function.
