IGEM:Imperial/2010/XylE team: Difference between revisions
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! Week 7 !! Monday !! Tuesday!! Wednesday !! Thursday !! Friday !! Weekend | ! Week 7 !! Monday !! Tuesday!! Wednesday !! Thursday !! Friday !! Weekend | ||
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| MORNING || 3A assemply: | | MORNING || | ||
ligation of RBS-XyL, J23101 promoter and PSB1C3 vector (overnight) | *3A assemply: ligation of RBS-XyL, J23101 promoter and PSB1C3 vector (overnight) | ||
|| *estimated date of primers delivery | ||''' *estimated date of primers delivery''' | ||
3A assemply: transformation of E.coli with XylE construct (overnight) | *3A assemply: transformation of E.coli with XylE construct (overnight) | ||
|| | || | ||
3A assemply: make replica plates (overnight) | *3A assemply: make replica plates (overnight) | ||
Catechol assay of E.coli | *Catechol assay of E.coli | ||
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*Mini-prep XylE E.coli | |||
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*Midi-prep XylE E.coli | |||
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| lunch break || || || || || || | | lunch break || || || || || || | ||
|- | |- | ||
| AFTERNOON || | | AFTERNOON || || | ||
* Starting | *Starting construction of XylE fusion protein | ||
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==Lab Notes== | ==Lab Notes== |
Revision as of 10:04, 12 August 2010
XylE team Lab Objectives
- construction of XylE fusion protein
- Testing expression of XylE in E.coli and characterization under the control of a constitutive promoter
- Construction of the -ComE promoter/XylE fussion protein- expression system
- Construction of the -LacI promoter/XylE fusion construct- expression system
Schedule
Week 6 | Monday | Tuesday | Wednesday | Thursday | Friday | Weekend |
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MORNING |
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lunch break | ||||||
AFTERNOON |
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|
Week 7 | Monday | Tuesday | Wednesday | Thursday | Friday | Weekend |
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MORNING |
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*estimated date of primers delivery
|
|
|
|
|
lunch break | ||||||
AFTERNOON |
|