IGEM:Imperial/Results/J37015
From OpenWetWare
11/8/06
Aims
Due to difficulty in both the PCR screen and Mini-preping of the final J37015 construct it was decided to test the cells for production of AHL, which should be produced in large quantities if the ligations had been successful.
Results
- Data File:
Comment
- From the results the T9002 Control actually has a greater Fluorescence/OD490 than the T9002 resuspended in J37015 supernatent
- This could be due to the fact that the T9002 cells that were used in the J37015 assay had to be spun down whereas the control cells were not
- The T9002 J37015 assay cells were also in the fridge for a period of around 2 hours due to a protocol error. This could have effected the cells ability to produce GFP.
- The results do not indicate any AHL at all in the supernatent of J37015 culture
- This possibily indicates the ligation wasn not successful
- However, due to the reasons above, it may simply be that being in the fridge/spun prevented the T9002 cells from responding to AHL
- General recomendation to change protocol so that both the Assay and Control T9002 cells are spun and resuspended in media
