IGEM:Imperial/Results/S01656: Difference between revisions
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The IPTG has '''not''' induced the production of a protein which weighs 28736 kd this should be loacted inside the oval. We are repeating the expperiment to confirm the results. We are sequencing the part to confirm the results. The ladder is invitrogen marker 12. | The IPTG has '''not''' induced the production of a protein which weighs 28736 kd ''(aiiA)'' this should be loacted inside the oval. We are repeating the expperiment to confirm the results. We are sequencing the part to confirm the results. The ladder is invitrogen marker 12. | ||
[[User:JohnChattaway|JohnChattaway]] 10:13, 23 August 2006 (EDT) | [[User:JohnChattaway|JohnChattaway]] 10:13, 23 August 2006 (EDT) | ||
Revision as of 07:19, 23 August 2006
S01656 Protocol Page
23rd August
Results of western Blot
The IPTG has not induced the production of a protein which weighs 28736 kd (aiiA) this should be loacted inside the oval. We are repeating the expperiment to confirm the results. We are sequencing the part to confirm the results. The ladder is invitrogen marker 12.
JohnChattaway 10:13, 23 August 2006 (EDT)
Sunday, 20 August 2006
- Normalised Fluorescence of +IPTG +AHL
- Tube 1 - 233
- Tube 2 - 237
- Tube 3 - 239
- Normalised Fluorescence of +IPTG -AHL
- Tube 1 - 219
- Normalised Fluorescence of -IPTG +AHL
- Tube 1 - 244
- Normalised Fluroescence of -IPTG -AHL
- Tube 1 - 218
- Comparison between +IPTG +AHL and -IPTG +AHL:
- % Difference = 2.94%
- Conclusion: Not statistically significant
- Resolution
- Test expression of AiiA protein by SDS electrophoresis
- Re-test the protocol on Tuesday 22 August 2006
Tuesday, 22 August 2006
- Different formulation used to calculate normalised fluorescence. Used the same calculations as the T9002. See updated spreadsheet.
- Normalised Fluorescence of +IPTG +AHL
- Tube 1 - 4409
- Tube 2 - 4659
- Tube 3 - 4574
- Normalised Fluorescence of +IPTG -AHL
- Tube 1 - 4574
- Normalised Fluorescence of -IPTG +AHL
- Tube 1 - 4769
- Tube 2 - 4762
- Tube 3 - 4834
- Normalised Fluroescence of -IPTG -AHL
- Tube 1 - 4445
- Comparison between +IPTG +AHL and -IPTG +AHL:
- % Difference = 4.25%
- Conclusion: Not statistically significant
- Resolution
- Test expression of AiiA protein by SDS electrophoresis
- Re-test the protocol on Wednesday 23 August 2006 with higher concentration of AHL
