IGEM:Imperial/Results/S01656

Sunday, 20 August 2006

• Normalised Fluorescence of +IPTG +AHL

Tube 1 - 233

Tube 2 - 237

Tube 3 - 239

• Normalised Fluorescence of +IPTG -AHL

Tube 1 - 219

• Normalised Fluorescence of -IPTG +AHL

Tube 1 - 244

• Normalised Fluroescence of -IPTG -AHL

Tube 1 - 218

• Comparison between +IPTG +AHL and -IPTG +AHL:

% Difference = 2.94%

Conclusion: Not statistically significant

• Resolution

Test expression of AiiA protein by SDS electrophoresis

Re-test the protocol on Tuesday 22 August 2006

Tuesday, 22 August 2006

• Different formulation used to calculate normalised fluorescence. Used the same calculations as the T9002. See updated spreadsheet.

• Normalised Fluorescence of +IPTG +AHL

Tube 1 - 4409

Tube 2 - 4659

Tube 3 - 4574

• Normalised Fluorescence of +IPTG -AHL

Tube 1 - 4574

• Normalised Fluorescence of -IPTG +AHL

Tube 1 - 4769

Tube 2 - 4762

Tube 3 - 4834

• Normalised Fluroescence of -IPTG -AHL

Tube 1 - 4445

• Comparison between +IPTG +AHL and -IPTG +AHL:

% Difference = 4.25%

Conclusion: Not statistically significant

• Resolution

Test expression of AiiA protein by SDS electrophoresis

Re-test the protocol on Wednesday 23 August 2006 with higher concentration of AHL