IGEM:JohnsHopkins/2008/Budget

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m (IGEM:JohnsHopkins/2008/Ideas/Budget moved to IGEM:JohnsHopkins/2008/Budget: Moved from Ideas "folder" to JHU team page)
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Ready for you to update James or Tejas
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Item'''
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| align="center" style="background:#f0f0f0;"|'''Supplier'''
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| align="center" style="background:#f0f0f0;"|'''Amount'''
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| align="center" style="background:#f0f0f0;"|'''[ ] if app'''
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| align="center" style="background:#f0f0f0;"|'''Quantity'''
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| align="center" style="background:#f0f0f0;"|'''Cat. #'''
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| align="center" style="background:#f0f0f0;"|'''Cost'''
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|-
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| ||||||||||||
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|-
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| DNA synthesis||||||||||||$4,000
 +
|-
 +
| ||||||||||Subtotal||4,000
 +
|-
 +
| PCR supplies||||||||||||
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|-
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| Taq pol||NEB||4000 units||5000 units/ml||1||M0273X||400
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|-
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| Standard Taq Reaction Buffer||NEB||6mL||10X||4||B9014S||40
 +
|-
 +
| 96-Well Nonskirted PCR Plates||Fisherbrand||25 plates||||2||08-408-220||143.58
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|-
 +
| 8-well; 0.2mL; Color: Natural||Fisherbrand||125 tubes||||2||08-408-237||316.62
 +
|-
 +
| Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source ||Sigma||5g||||1||A3377||55.4
 +
|-
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| Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powder||Sigma||100mg||||1||G8877||95
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|-
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| Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC)  ||Sigma||100mg||||1||30320||62.7
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|-
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| Thymidine 5′-triphosphate sodium salt ~97%||Sigma||50mg||||1||T0251||118
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|-
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| ||||||||||||
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|-
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| ||||||||||Subtotal||1,231
 +
|-
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| ||||||||||||
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|-
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| Culturing Supplies||||||||||||
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|-
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| Tryptone Peptone(Bacto-Tryptone)||Difco||2kg||||1||211699||263.9
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|-
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| Yeast Extract||Difco||2kg||||1||212720||326.9
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|-
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| NaCl||Sigma||1kg||||1||S6191 ||53.8
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|-
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| Dextrose (D-Glucose)||J.T Baker||12 KG||||1||1920-07||457.82
 +
|-
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| Potassium Chloride||Sigma||1 kg||||1||P9541 ||49.5
 +
|-
 +
| Magnesium Sulfate (anhydrous)||Sigma||100g||||1||246972 ||23.4
 +
|-
 +
| Peptone, Bacto||Difco||2kg||||1||211820||286.7
 +
|-
 +
| Bacto-Agar||Difco||2kg||||1||214030||658.05
 +
|-
 +
| YNB w/o Amino Acids||Difco||100g||||1||291940||79.6
 +
|-
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|  96-Well Plates Sterile||Fisherbrand||40 plates||||1||21-377-205||208
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|-
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| Raised Ridge; 100 O.D. x 15mmH Petri Dishes||Fisherbrand||500 plates||||2||08-757-12||300.1
 +
|-
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| Ampicillin anhydrous, 96.0-100.5% (anhydrous basis)  ||Sigma||25g||||1||A9393||157
 +
|-
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|  G 418 disulfate salt powder, cell culture tested||Sigma||1g||||1||A1720||88.9
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|-
 +
| ||||||||||||
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|-
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| ||||||||||||
 +
|-
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| ||||||||||Subtotal||2953.67
 +
|-
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| ||||||||||||
 +
|-
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| ||||||||||||
 +
|-
 +
| ||||||||||||
 +
|-
 +
| ||||||||||||
 +
|-
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| ||||||||||||
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|-
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| Gel Electrophoresis supplies||||||||||||
 +
|-
 +
| EDTA||J.T Baker||500g||||1||8994-01||212.5
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|-
 +
| 2-Log DNA Ladder||NEB||500 μg||1,000 μg/ml||1||N3200L||244
 +
|-
 +
| Xylene Cyanol||Sigma||10g||||1||X4126 ||40.8
 +
|-
 +
| Bromophenol Blue||Sigma||5g||||1||B8026||24.3
 +
|-
 +
| Glycerol||Sigma||500mL||||1||G5516||31.2
 +
|-
 +
| Ethidium Bromide||Sigma||5g||||1||E7637||105
 +
|-
 +
| Taurine||Sigma||1kg||||1||T0625 ||403
 +
|-
 +
| Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENT||J.T Baker||500g||||1||A426-07||651.5
 +
|-
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| Tris -Base||J.T Baker||5kg||||1||4109-06||363.85
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|-
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| ||||||||||||
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|-
 +
| ||||||||||||
 +
|-
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| ||||||||||Subtotal||2076.15
 +
|-
 +
|
 +
|}

Revision as of 00:06, 4 March 2008

Item Supplier Amount [ ] if app Quantity Cat. # Cost
DNA synthesis$4,000
Subtotal4,000
PCR supplies
Taq polNEB4000 units5000 units/ml1M0273X400
Standard Taq Reaction BufferNEB6mL10X4B9014S40
96-Well Nonskirted PCR PlatesFisherbrand25 plates208-408-220143.58
8-well; 0.2mL; Color: NaturalFisherbrand125 tubes208-408-237316.62
Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source Sigma5g1A337755.4
Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powderSigma100mg1G887795
Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC) Sigma100mg13032062.7
Thymidine 5′-triphosphate sodium salt ~97%Sigma50mg1T0251118
Subtotal1,231
Culturing Supplies
Tryptone Peptone(Bacto-Tryptone)Difco2kg1211699263.9
Yeast ExtractDifco2kg1212720326.9
NaClSigma1kg1S6191 53.8
Dextrose (D-Glucose)J.T Baker12 KG11920-07457.82
Potassium ChlorideSigma1 kg1P9541 49.5
Magnesium Sulfate (anhydrous)Sigma100g1246972 23.4
Peptone, BactoDifco2kg1211820286.7
Bacto-AgarDifco2kg1214030658.05
YNB w/o Amino AcidsDifco100g129194079.6
96-Well Plates SterileFisherbrand40 plates121-377-205208
Raised Ridge; 100 O.D. x 15mmH Petri DishesFisherbrand500 plates208-757-12300.1
Ampicillin anhydrous, 96.0-100.5% (anhydrous basis) Sigma25g1A9393157
G 418 disulfate salt powder, cell culture testedSigma1g1A172088.9
Subtotal2953.67
Gel Electrophoresis supplies
EDTAJ.T Baker500g18994-01212.5
2-Log DNA LadderNEB500 μg1,000 μg/ml1N3200L244
Xylene CyanolSigma10g1X4126 40.8
Bromophenol BlueSigma5g1B802624.3
GlycerolSigma500mL1G551631.2
Ethidium BromideSigma5g1E7637105
TaurineSigma1kg1T0625 403
Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENTJ.T Baker500g1A426-07651.5
Tris -BaseJ.T Baker5kg14109-06363.85
Subtotal2076.15



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