IGEM:JohnsHopkins/2008/Budget

Item	Supplier	Amount	[ ] if app	Quantity	Cat. #	Cost
DNA synthesis						$4,000
					Subtotal	4,000
PCR supplies
Taq pol	NEB	4000 units	5000 units/ml	1	M0273X	400
Standard Taq Reaction Buffer	NEB	6mL	10X	4	B9014S	40
96-Well Nonskirted PCR Plates	Fisherbrand	25 plates		2	08-408-220	143.58
8-well; 0.2mL; Color: Natural	Fisherbrand	125 tubes		2	08-408-237	316.62
Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source	Sigma	5g		1	A3377	55.4
Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powder	Sigma	100mg		1	G8877	95
Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC)	Sigma	100mg		1	30320	62.7
Thymidine 5′-triphosphate sodium salt ~97%	Sigma	50mg		1	T0251	118
					Subtotal	1,231
Culturing Supplies
Tryptone Peptone(Bacto-Tryptone)	Difco	2kg		1	211699	263.9
Yeast Extract	Difco	2kg		1	212720	326.9
NaCl	Sigma	1kg		1	S6191	53.8
Dextrose (D-Glucose)	J.T Baker	12 KG		1	1920-07	457.82
Potassium Chloride	Sigma	1 kg		1	P9541	49.5
Magnesium Sulfate (anhydrous)	Sigma	100g		1	246972	23.4
Peptone, Bacto	Difco	2kg		1	211820	286.7
Bacto-Agar	Difco	2kg		1	214030	658.05
YNB w/o Amino Acids	Difco	100g		1	291940	79.6
96-Well Plates Sterile	Fisherbrand	40 plates		1	21-377-205	208
Raised Ridge; 100 O.D. x 15mmH Petri Dishes	Fisherbrand	500 plates		2	08-757-12	300.1
Ampicillin anhydrous, 96.0-100.5% (anhydrous basis)	Sigma	25g		1	A9393	157
G 418 disulfate salt powder, cell culture tested	Sigma	1g		1	A1720	88.9
					Subtotal	2953.67
Gel Electrophoresis supplies
EDTA	J.T Baker	500g		1	8994-01	212.5
2-Log DNA Ladder	NEB	500 μg	1,000 μg/ml	1	N3200L	244
Xylene Cyanol	Sigma	10g		1	X4126	40.8
Bromophenol Blue	Sigma	5g		1	B8026	24.3
Glycerol	Sigma	500mL		1	G5516	31.2
Ethidium Bromide	Sigma	5g		1	E7637	105
Taurine	Sigma	1kg		1	T0625	403
Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENT	J.T Baker	500g		1	A426-07	651.5
Tris -Base	J.T Baker	5kg		1	4109-06	363.85
					Subtotal	2076.15
					Grand total	10,261

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