IGEM:JohnsHopkins/2008/Budget

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Current revision (01:10, 4 March 2008) (view source)
 
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| align="center" style="background:#f0f0f0;"|'''Cost'''
| align="center" style="background:#f0f0f0;"|'''Cost'''
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| Restriction Enzymes||||||||||||$1,500
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|-
| DNA synthesis||||||||||||$4,000  
| DNA synthesis||||||||||||$4,000  
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| ||||||||||Subtotal||4,000
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| ||||||||||Subtotal||5,500
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| PCR supplies||||||||||||
| PCR supplies||||||||||||
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| ||||||||||Grand total||10,261
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| ||||||||||Grand total||11,761
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Current revision

Item Supplier Amount [ ] if app Quantity Cat. # Cost
Restriction Enzymes$1,500
DNA synthesis$4,000
Subtotal5,500
PCR supplies
Taq polNEB4000 units5000 units/ml1M0273X400
Standard Taq Reaction BufferNEB6mL10X4B9014S40
96-Well Nonskirted PCR PlatesFisherbrand25 plates208-408-220143.58
8-well; 0.2mL; Color: NaturalFisherbrand125 tubes208-408-237316.62
Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source Sigma5g1A337755.4
Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powderSigma100mg1G887795
Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC) Sigma100mg13032062.7
Thymidine 5′-triphosphate sodium salt ~97%Sigma50mg1T0251118
Subtotal1,231
Culturing Supplies
Tryptone Peptone(Bacto-Tryptone)Difco2kg1211699263.9
Yeast ExtractDifco2kg1212720326.9
NaClSigma1kg1S6191 53.8
Dextrose (D-Glucose)J.T Baker12 KG11920-07457.82
Potassium ChlorideSigma1 kg1P9541 49.5
Magnesium Sulfate (anhydrous)Sigma100g1246972 23.4
Peptone, BactoDifco2kg1211820286.7
Bacto-AgarDifco2kg1214030658.05
YNB w/o Amino AcidsDifco100g129194079.6
96-Well Plates SterileFisherbrand40 plates121-377-205208
Raised Ridge; 100 O.D. x 15mmH Petri DishesFisherbrand500 plates208-757-12300.1
Ampicillin anhydrous, 96.0-100.5% (anhydrous basis) Sigma25g1A9393157
G 418 disulfate salt powder, cell culture testedSigma1g1A172088.9
Subtotal2953.67
Gel Electrophoresis supplies
EDTAJ.T Baker500g18994-01212.5
2-Log DNA LadderNEB500 μg1,000 μg/ml1N3200L244
Xylene CyanolSigma10g1X4126 40.8
Bromophenol BlueSigma5g1B802624.3
GlycerolSigma500mL1G551631.2
Ethidium BromideSigma5g1E7637105
TaurineSigma1kg1T0625 403
Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENTJ.T Baker500g1A426-07651.5
Tris -BaseJ.T Baker5kg14109-06363.85
Subtotal2076.15
Grand total11,761



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