IGEM:JohnsHopkins/2008/Budget
From OpenWetWare
| Item | Supplier | Amount | [ ] if app | Quantity | Cat. # | Cost |
| Restriction Enzymes | $1,500 | |||||
| DNA synthesis | $4,000 | |||||
| Subtotal | 5,500 | |||||
| PCR supplies | ||||||
| Taq pol | NEB | 4000 units | 5000 units/ml | 1 | M0273X | 400 |
| Standard Taq Reaction Buffer | NEB | 6mL | 10X | 4 | B9014S | 40 |
| 96-Well Nonskirted PCR Plates | Fisherbrand | 25 plates | 2 | 08-408-220 | 143.58 | |
| 8-well; 0.2mL; Color: Natural | Fisherbrand | 125 tubes | 2 | 08-408-237 | 316.62 | |
| Adenosine 5′-triphosphate disodium salt Grade II, ≥99%, crystalline, from microbial source | Sigma | 5g | 1 | A3377 | 55.4 | |
| Guanosine 5′-triphosphate sodium salt hydrate ≥95% (HPLC), powder | Sigma | 100mg | 1 | G8877 | 95 | |
| Cytidine 5\'-triphosphate disodium salt hydrate BioChemika, ≥90.0% (HPLC) | Sigma | 100mg | 1 | 30320 | 62.7 | |
| Thymidine 5′-triphosphate sodium salt ~97% | Sigma | 50mg | 1 | T0251 | 118 | |
| Subtotal | 1,231 | |||||
| Culturing Supplies | ||||||
| Tryptone Peptone(Bacto-Tryptone) | Difco | 2kg | 1 | 211699 | 263.9 | |
| Yeast Extract | Difco | 2kg | 1 | 212720 | 326.9 | |
| NaCl | Sigma | 1kg | 1 | S6191 | 53.8 | |
| Dextrose (D-Glucose) | J.T Baker | 12 KG | 1 | 1920-07 | 457.82 | |
| Potassium Chloride | Sigma | 1 kg | 1 | P9541 | 49.5 | |
| Magnesium Sulfate (anhydrous) | Sigma | 100g | 1 | 246972 | 23.4 | |
| Peptone, Bacto | Difco | 2kg | 1 | 211820 | 286.7 | |
| Bacto-Agar | Difco | 2kg | 1 | 214030 | 658.05 | |
| YNB w/o Amino Acids | Difco | 100g | 1 | 291940 | 79.6 | |
| 96-Well Plates Sterile | Fisherbrand | 40 plates | 1 | 21-377-205 | 208 | |
| Raised Ridge; 100 O.D. x 15mmH Petri Dishes | Fisherbrand | 500 plates | 2 | 08-757-12 | 300.1 | |
| Ampicillin anhydrous, 96.0-100.5% (anhydrous basis) | Sigma | 25g | 1 | A9393 | 157 | |
| G 418 disulfate salt powder, cell culture tested | Sigma | 1g | 1 | A1720 | 88.9 | |
| Subtotal | 2953.67 | |||||
| Gel Electrophoresis supplies | ||||||
| EDTA | J.T Baker | 500g | 1 | 8994-01 | 212.5 | |
| 2-Log DNA Ladder | NEB | 500 μg | 1,000 μg/ml | 1 | N3200L | 244 |
| Xylene Cyanol | Sigma | 10g | 1 | X4126 | 40.8 | |
| Bromophenol Blue | Sigma | 5g | 1 | B8026 | 24.3 | |
| Glycerol | Sigma | 500mL | 1 | G5516 | 31.2 | |
| Ethidium Bromide | Sigma | 5g | 1 | E7637 | 105 | |
| Taurine | Sigma | 1kg | 1 | T0625 | 403 | |
| Agarose, Standard, Low Electroendosmosis (EEO), ULTRAPURE BIOREAGENT | J.T Baker | 500g | 1 | A426-07 | 651.5 | |
| Tris -Base | J.T Baker | 5kg | 1 | 4109-06 | 363.85 | |
| Subtotal | 2076.15 | |||||
| Grand total | 11,761 | |||||
