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(New page: Bio-Battery Budget Year One Determine which Photosynthesis Candidate shows the most promise (characteristically) for the bio-battery Chlamydomonas reinhardtii Chlorella Vulgaris V...)
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Determine which Photosynthesis Candidate shows the most promise (characteristically) for the bio-battery Chlamydomonas reinhardtii Chlorella Vulgaris Volvox Halobacterium Purple Photosynthetic Bacteria (like Rhodospirillia) (produce no oxygen, some use hydrogen) Other Cyanobacteria
Summarize useful pathways in Rhodoferax Ferrireducens by studying genome and annotations. Use information gained from bioinformatics work to predict problems that will present themselves in this battery system, from the perspective of organism incompatibility and lack of versatility. Design genetics systems that could be used to engineer these organisms to be better compatible with each other and more versatile.
Stipend: $12,000 for five students
Total for year one: $12,000
Test which Photosynthetic Candidate will work best with solution of Rhodoferrax Ferrireducens. Test growth in 100% CO2 environment, low atmospheric pressure, high acetate concentration Test components of iron-reducing pathway in R. Ferrireducens and filling in gaps from genome study. (Example, inhibited conditions) Test growth in 100% CO2, low atmospheric pressure, high acetate concentration, high oxidized acetate concentration, high oxygen content (Stipend: $12,000 for five students) Cost for supplies: $500 for organisms $1500 for growth media (salts, sugar, etc.) $1500 for Materials for constructing controlled atmospheric environment, pumps, and CO2 cartridges $4500 for spec analysis of growth (We may need to get our own spectrophotometer. It will be useful to have it in the future as well for synthetic biology.)
Total for year one: $20,000
Use information gained from bioinformatic and experimental work from previous two years to further design and modify potential genetic systems to improve organism compatibility and versatility.
Design genes that will produce and release acetate into solution from photosynthetic organism. Express said genes in photosynthetic organism and determine change in growth levels. Also, determine changes in level or iron reduction.
Stipend: $12,000 for five students Cost of Supplies: $2000 for various battery components $1500 for growth media (salt, sugar, etc.) $1500 for enzymes $4000 for DNA synthesis
Total for year three: $21,000
Explore possible cathode materials. Determine what cathodes can be readily oxidized, with the reduced component accessible to the photosystem of the photosynthetic organism. Test organisms' compatibilities with cathode.
Do the same for the iron anode.
Combine systems into a rechargeable biobattery. Test for voltage, current, changes in growth and sustainability.
Total for year four: $17,000 - $19,000
Use data gained from previous year to determine what are the most significant problems that are occurring and why they are occurring.
Fine tune efficiency and usability, and perform error corrections. E.g.: Improve growth conditions for both organisms by addition of new genes that prevent impedance to growth that would naturally occur in such a system. Add/modify genes to provide user with greater, finer control over system.
Total for year five: $18,000 - $22,000
Use this time space to further improve and adjust system. Then present system at iGEM jamboree.
Cost to attend iGEM competition: $5,000 Stipend: $12,000 for five students Additional supplies: ~$3,000
Total for year six: ~$20,000
Total for six years: ~$108,000 - $112,000