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| Bio-Battery Budget
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| Year One
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| Determine which Photosynthesis Candidate shows the most promise (characteristically) for the bio-battery
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| Chlamydomonas reinhardtii
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| Chlorella Vulgaris
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| Volvox
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| Halobacterium
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| Purple Photosynthetic Bacteria (like Rhodospirillia) (produce no oxygen, some use hydrogen)
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| Other Cyanobacteria
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| Summarize useful pathways in Rhodoferax Ferrireducens by studying genome and annotations.
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| Use information gained from bioinformatics work to predict problems that will present themselves in this battery system, from the perspective of organism incompatibility and lack of versatility.
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| Design genetics systems that could be used to engineer these organisms to be better compatible with each other and more versatile.
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| Stipend: $12,000 for five students
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| Total for year one: $12,000
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| Year two
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| Test which Photosynthetic Candidate will work best with solution of Rhodoferrax Ferrireducens.
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| Test growth in 100% CO2 environment, low atmospheric pressure, high acetate concentration
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| Test components of iron-reducing pathway in R. Ferrireducens and filling in gaps from genome study. (Example, inhibited conditions)
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| Test growth in 100% CO2, low atmospheric pressure, high acetate concentration, high oxidized acetate concentration, high oxygen content
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| (Stipend: $12,000 for five students)
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| Cost for supplies:
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| $500 for organisms
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| $1500 for growth media (salts, sugar, etc.)
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| $1500 for Materials for constructing controlled atmospheric environment, pumps, and CO2 cartridges
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| $4500 for spec analysis of growth (We may need to get our own spectrophotometer. It will be useful to have it in the future as well for synthetic biology.)
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| Total for year one: $20,000
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| Year three
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| Use information gained from bioinformatic and experimental work from previous two years to further design and modify potential genetic systems to improve organism compatibility and versatility.
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| Design genes that will produce and release acetate into solution from photosynthetic organism.
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| Express said genes in photosynthetic organism and determine change in growth levels.
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| Also, determine changes in level or iron reduction.
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| Stipend: $12,000 for five students
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| Cost of Supplies:
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| $2000 for various battery components
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| $1500 for growth media (salt, sugar, etc.)
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| $1500 for enzymes
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| $4000 for DNA synthesis
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| Total for year three: $21,000
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| Year four
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| Explore possible cathode materials. Determine what cathodes can be readily oxidized, with the reduced component accessible to the photosystem of the photosynthetic organism.
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| Test organisms' compatibilities with cathode.
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| Do the same for the iron anode.
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| Combine systems into a rechargeable biobattery. Test for voltage, current, changes in growth and sustainability.
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| Total for year four: $17,000 - $19,000
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| Year five
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| Use data gained from previous year to determine what are the most significant problems that are occurring and why they are occurring.
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| Fine tune efficiency and usability, and perform error corrections.
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| E.g.: Improve growth conditions for both organisms by addition of new genes that prevent impedance to growth that would naturally occur in such a system.
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| Add/modify genes to provide user with greater, finer control over system.
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| Total for year five: $18,000 - $22,000
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| Year six
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| Use this time space to further improve and adjust system. Then present system at iGEM jamboree.
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| Cost to attend iGEM competition: $5,000
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| Stipend: $12,000 for five students
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| Additional supplies: ~$3,000
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| Total for year six: ~$20,000
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| Total for six years: ~$108,000 - $112,000
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