IGEM:Kyoto/2008

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our team logo NOW PRINTING... Project Coming soon! X Y Next Meeting On 10th MAY 13:00 農学生命F1セミナー室2 Contact us Email:		Students Now updating... Instructors Hirohide Saito Shin-ichiro M. Nomura Masahiro Takinoue
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		Protocols Escherichia coli [show][hide] Preparing chemically competent cells Electrocompetent cells Miniprep Electroporation Colony PCR Bacterial cell culture Beta-Galactosidase Assay (A better Miller) Miniprep/Qiagen kit Miniprep/Kit-free high-throughput protocol Transforming chemically competent cells Bacterial transformation Making a long term stock of bacteria P1vir phage transduction Preparing chemically competent cells (Inoue) Transforming chemically competent cells (Inoue) One step 'miniprep' method for the isolation of plasmid DNA TOP10 chemically competent cells Miniprep/GET buffer Maxiprep of plasmid DNA from E. coli Recombineering/Lambda red-mediated gene replacement ChIP-Chip E. coli Engineering BioBrick vectors from BioBrick parts/Colony PCR Cell counting/plating Cell and tissue lysis hub Cell cycle analysis In-fusion biobrick assembly Miniprep/TENS miniprep Knight:Colony PCR Endy:Colony PCR Endy:E. coli Western Blot Endy:STN Chemostat Protocol Knight:Preparing electrocompetent cells Knight:Electroporation Belcher/Knight: Electrocompetent Cells Lory:Quick transformant screen Seth:Quick Transformant Screen Sauer:Lysing E. coli with Lysozymes Knight:DNA Spots Knight:Miniprep low copy plasmids Knight:Beta-galactosidase assay Knight:Beta-galactosidase assay/96 well format Knight:Beta-galactosidase assay/Fluorescence Knight:Preparing stabs Duffy:LIC Cloning Huang: Agarose Slides for Imaging Richard Lab:Electroporation of E. coli Richard Lab:Preparing electrocompetent cells Erman's Lab:DNA Miniprep with Alkaline Lysis McClean:Competent Cells Gill:Rapid bacterial DNA prep Nucleic acids [show][hide] Agarose gel electrophoresis Phenol/chloroform extraction Ethanol precipitation of nucleic acids Quantitating nucleic acids PCR techniques Real-time PCR QRT-PCR Q-PCR Bioanalyzer Alm:Agarose gel electrophoresis DIYbio:Notebook/Keiki Gels DNA [show][hide] Agarose gel electrophoresis DNA ligation Yeast DNA Prep Ethanol precipitation of small DNA fragments Purification of DNA Annealing primers PNK Treatment of DNA Ends Miniprep Designing primers Phosphatase treatment of linearized vector Site-directed mutagenesis Restriction digest Phenol/chloroform extraction Sequencing DNA PCR Ethanol precipitation of nucleic acids 'Round-the-horn site-directed mutagenesis Library Generation Resuspension of primers Miniprep/Qiagen kit Miniprep/Kit-free high-throughput protocol One step 'miniprep' method for the isolation of plasmid DNA Cloning DNA Synthesis from Oligos PCR Overlap Extension Miniprep/GET buffer Electrophoretic mobility shift assay Centrifugal filtration/Nucleic acids Affymetrix DNA labelling for gene expression arrays Annealing and primer extension TempliPhi Quantitating nucleic acids Maxiprep of plasmid DNA from E. coli PCR techniques Real-time PCR QRT-PCR Anneal Extend PCR Protocol - for linking 2 pieces of DNA Streptavidin purification of DNA fragments DNA Precipitation Q-PCR Cloning and sequencing Engineering BioBrick vectors from BioBrick parts/DNA ligation Engineering BioBrick vectors from BioBrick parts/Dephosphorylation Engineering BioBrick vectors from BioBrick parts/Restriction digest Assembly pcr Cloning Checklist Bioanalyzer Size selective DNA precipitation SB DNA blot (Southern) Polyacrylamide gel electrophoresis DNA Seq Prep Cloning Protocol DNA Quantification Rapid plant gDNA extraction Amplified insert assembly Miniprep/TENS miniprep MIT:Sequencing BioBrick DNA Endy:Templiphi Knight:Micropure EZ and Microcon purification Knight:Annealing and primer extension with Klenow polymerase Knight:DNA ligation using NEB Quick Ligation Kit Knight:Restriction Digest Knight:Site-directed mutagenesis/Single site Silver: PCR Knight:Agarose gel electrophoresis Knight:TOPO TA cloning Endy:Double stranding oligo libraries Knight:Sequencing DNA Knight:Annealing and primer extension with Taq polymerase Knight:Reconstituting primers Kafatos:Minipreps "Core Sample" PCR: Amplifying DNA from agarose gels Knight:Electrophoretic mobility shift assay Jamesh008:consensus DNA ligation protocol Lory:Quick transformant screen Knight:Maxiprep Knight:PhoenIX Maxiprep Kit Seth:Quick Transformant Screen Knight:TempliPhi Sauer:Stitching Genes by PCR Streptomyces:Protocols/DNA Digest Etchevers:ChIP francais Knight:Site-directed mutagenesis/Multi site Knight:Miniprep low copy plasmids Alm:Agarose gel electrophoresis Knight:DNA ligation using T4 DNA ligase NanoBio: PCR Sacks:RAD-seq Duffy:LIC Cloning Weiss Lab:DNA labeling DIYbio:Notebook/Keiki Gels Richard Lab:Agarose Gel Electrophoresis Restriction Digest: Partial Richard Lab:Restriction Digest Richard Lab:Amplified insert assembly Corum:PCR Corum:digest Corum:T4 Ligation Corum:DNA Digestion Corum:DNA Hybridization Corum:Digestion Corum:Whole Plasmid PCR Brennan:Miniprep for vaccinia virus DNA isolation RNA [show][hide] Agarose gel electrophoresis RNA extraction Probe Prep, 32P End-Labeled Probes In vitro transcription with T7 RNA polymerase Phenol/chloroform extraction Ethanol precipitation of nucleic acids RNase Protection Assay Northern Blot, 32P End-Labeled Probes RNA Half-life Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing RNA Quality Control Affymetrix Target Hybridization Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing RNA electrophoresis Quantitating nucleic acids PCR techniques Real-time PCR QRT-PCR/Single tube QRT-PCR QRT-PCR/Two tubes Q-PCR RNA extraction using trizol/tri Bioanalyzer RNA blot (Northern) Making RNA probes with T7 transcription Endy:Northern Blot, 32P End-Labeled Probes Endy:RNA extraction Knight:In vitro transcription Knight:RNA electrophoresis/Denaturing Knight:RNA electrophoresis Knight:RNA electrophoresis/Native Etchevers:Extraction ARN francais Sauer:RNA Purification from E. coli Alm:Agarose gel electrophoresis Endy:Northern blot, AlkPhos end-labeled probes Dahlquist:DNA Microarray Protocol Griffin:Nested RT-PCR DIYbio:Notebook/Keiki Gels Protein [show][hide] Beta-galactosidase assay Acrylamide gels Silver staining Purification of His-tagged proteins Free Sulfhydryl Determination Antigen retrieval Electrophoretic mobility shift assay Protein blot (Western) hub Chromatin Immunoprecipitation Dot Blot User:Leprevost/Purification of His-tagged proteins/Denaturing Bioanalyzer Isolate leydig cells from testes Lactobacillus planarum miniprep Protein Purification - Salting Out Feruloyl Esterase Protocols Beta-galactosidase Screen Beta-glucuronidase protocols User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/05/23 SDS-PAGE sample buffer (Morris formulation) Endy:E. coli Western Blot Sauer:Expression/purification of 35S-Met proteins Silver: Lysate for Western Silver: Coomassie Stain Silver: Destain Keating:Experimental Protocols:HPLC Knight:Purification of His-tagged proteins/Denaturing Knight:NuPAGE electrophoresis Knight:Electrophoretic mobility shift assay Knight:Purification of His-tagged proteins Knight:Purification of His-tagged proteins/Native Knight:Protein solubility Knight:Purification of His-tagged proteins/Denaturing with refolding Knight:Centrifuge desalting Knight:Centrifuge desalting/Zeba columns Knight:NuPAGE electrophoresis/Fast staining Knight:NuPAGE electrophoresis/Slow staining Knight:NuPAGE electrophoresis/Hybrid staining Sauer:Lysing E. coli with Lysozymes Knight:NuPAGE electrophoresis/Gel drying Etchevers:ChIP francais Alm:Agarose gel electrophoresis ISISBio:Protocols/p11 resin preparation ISISBio:Protocols/Sortase mediated ligation ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation ISISBio:Protocols/Sortase mediated ligation/Solid support ligation ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation Griffin: Ultimate Immunoprecipitation Guide Griffin:Nuclear Extract Preparation Griffin:Western Blot Optimization Griffin:Immunohistochemistry Paraffin Griffin:Membrane Stripping and Reprobing Griffin:Antibody Elution Buffers Griffin:In Vitro Kinase Assay Griffin:Antibody Related Solutions & Recipes Griffin:Isolate Leukocytes from whole blood Fong:Bradford protein assay Prbbbb:small scale expression v1 Prbbbb:Coomassi Ethanol stain Prbbbb:inclusion body solubilization v1 Prbbbb:in vitro FRET FRB FKBP v1 iGEM iGEM 2008 iGEM 2007 Search <sitesearch>title=Search this team!</sitesearch>
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