IGEM:MIT/2005/Actuator: Difference between revisions
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2. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7029306&query_hl=8 An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.] | 2. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7029306&query_hl=8 An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.] | ||
I haven't gotten past the abstract, but it looks like these kids secreted proinsulin. They "tagged" there proinsulin by using a preproinsulin sequence. Preproinsulin is proinsulin with some amino acids typically used to move the protein out of the roughER (golgiapp.) and is immediately cleaved in prokos. In e.coli, the pre_sequence convinces the membrane to let the protein out. Can't stomach the paper yet though, and would really like to find how the sequence was | I haven't gotten past the abstract, but it looks like these kids secreted proinsulin. They "tagged" there proinsulin by using a preproinsulin sequence. Preproinsulin is proinsulin with some amino acids typically used to move the protein out of the roughER (golgiapp.) and is immediately cleaved in prokos. In e.coli, the pre_sequence convinces the membrane to let the protein out. Can't stomach the paper yet though, and would really like to find how the sequence was promoted in the first place. These kid's were not trying to control the rate of insulin. I am. The DNA sequence written in the paper seems incomplete (short) - maybe it refers to another paper for the complete insulin sequence. Soon I will compare this paper's insulin to the one we got from invitrogen (or whatever). I wish that was wikified. | ||
Revision as of 19:39, 20 June 2005
The actuator team actually consists of maxiney and bosworth. Some pops signal is given to us via the the other igemers, and we ought to be able to couple it to any output system imagineable, prove that the system works, and maybe just maybe come up with elegant ways to quantify the phenomena. Short Term:
- choose a flourescent protein (people seem opinionated on this!) and spec out the requirements to make it flouresce to aid the sensor and s.p. group's initial system designs.
- lay groundwork research for making e.coli spit out a fashionable form of insulin. Also, design tests to show presence of insulin and show world that "we meant to do that."
Some insulin Papers. One of our local wisemen, Drew Endy, noted that the apparent world expert on the topic of insulin and e.coli at one point went by the name of Karen Talmadge. She used to hang out at Harvard. Now she runs some serious biotech stuff, but in the 80's she was a postdoc doing some pretty cool research that probably catalyzed her rise in the biotech industry. We read (present tense) her papers.
1. Eukaryotic signal sequence transports insulin antigen in Escherichia coli.
2. An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria. I haven't gotten past the abstract, but it looks like these kids secreted proinsulin. They "tagged" there proinsulin by using a preproinsulin sequence. Preproinsulin is proinsulin with some amino acids typically used to move the protein out of the roughER (golgiapp.) and is immediately cleaved in prokos. In e.coli, the pre_sequence convinces the membrane to let the protein out. Can't stomach the paper yet though, and would really like to find how the sequence was promoted in the first place. These kid's were not trying to control the rate of insulin. I am. The DNA sequence written in the paper seems incomplete (short) - maybe it refers to another paper for the complete insulin sequence. Soon I will compare this paper's insulin to the one we got from invitrogen (or whatever). I wish that was wikified.
