IGEM:MIT/2005/Experiments
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[[../Experimental Flowchart/]]
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Experiment Status
Jenny
Stock-up MiniPreps grow up B0015, J13002, E0840 overnight B0015, J13002, E0840 miniprep B0015, J13002, E0840 glycerol stock J13002
scFv.TT (J07012-14.B0015) digest B0015 E/X, recipient obtain digested scFvs E/S, prefix donor gel purify B0015 ligate J07012-14.B0015 transform overnights for miniprep identify correct clones (digest), streak plates overnights for freezer stock freezer stocks scFv.B0015
Promoter.RBS.scFv PCR start codon to scFv digest Promoter.RBS BB (recipient) obtain digested scFvs (suffix donor) gel purify promoter.RBS ligate promoter.RBS.svFv transform overnights for miniprep identify correct clones (digest), streak plates overnights for freezer stock freezer stocks promoter.RBS.scFv
scFvs: 4M5.3, S101A, 4420 biobrick ends digest scFv + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones (digest) overnights for freezer stock freezer stocks
FecA (full length) PCR bb ends digest FecA + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones overnights for freezer stock freezer stocks
Jessica
MalE PCR digest MalE + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones
PhoA PCR with higher annealing temperature digest PhoA+ pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones PhoA' QuikChange
Annie
FecA promoter PCR with current primers with higher annealing temperature PCR with newly designed primers digest FecApro + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecI PCR bb end digest FecI + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecR PCR bb end digest FecR + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecA'
QuikChange
FecR'
QuikChange
Project Experiments
Input Device: [[../Input: Ligand#Experiments/|fluorescein]] Head Receiver Unit: [[../Receiver Head Unit: scFv#Experiments/|anti-fluorescein scFv]] Receiver 1: [[../Receiver 1: ToxR#Experiments/|ToxR]] Receiver 2: [[../Sensor 2#Experiments/|FecA]] Signal Processor: [[../Signal processing#Experiments/|Exp. Here]] Actuator: [[../Actuator#Experiments/|Exp. Here]]
[[../Experimental Timeline/]]