IGEM:MIT/2005/Ordered Orders ...: Difference between revisions

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  synthesized by Invitrogen
  synthesized by Invitrogen
  date ordered: July 14
  date ordered: July 14
  date received: - ~"a couple days"
  recieved.


  '''Receiver 2'''
  '''Receiver 2'''
Line 11: Line 11:
  synthesized by Invitrogen
  synthesized by Invitrogen
  date ordered: July 14
  date ordered: July 14
  date received: - ~"a couple days"
  received.


  '''Receiver 1'''
  '''Receiver 1'''
Line 17: Line 17:
  synthesized by Invitrogen
  synthesized by Invitrogen
  date ordered: July 14
  date ordered: July 14
  date received: - ~"a couple days"
  received.


  '''Receiver 1'''
  '''Receiver 1'''
Line 37: Line 37:
  Consists of pairs of complementary single stranded DNA, each connected to fluorescein
  Consists of pairs of complementary single stranded DNA, each connected to fluorescein
  date ordered: July 13
  date ordered: July 13
  date received: expected July 14-15--ask TK
  received.


  '''Receiver Head Unit: scFv'''
  '''Receiver Head Unit: scFv'''

Revision as of 08:14, 21 July 2005

Assemble stack chronologically. New entries on top. Recommend following template 

Receiver 1 & 2
scFv forward&reverse primers (for ToxR & FecA)
synthesized by Invitrogen
date ordered: July 14
recieved.

Receiver 2
FecA forward, reverse, and mutant removal primers
synthesized by Invitrogen
date ordered: July 14
received.

Receiver 1
malE and PhoA forward and reverse primers
synthesized by Invitrogen
date ordered: July 14
received.

Receiver 1
CTX Promoter - part# BBa_J07007
on plasmid: 
synthesized by BlueHeron
date ordered: July 13
date received: - (~1-2weeks)

Receiver 1
ToxR' - part# BBa_J07009
on plasmid:
synthesized by BlueHeron
date ordered: July 13
date received: - (~1-2 weeks)

Input: Ligand -> Receiver 1
Fluorescein molecules connected by a double-stranded DNA fragment
Consists of pairs of complementary single stranded DNA, each connected to fluorescein
date ordered: July 13
received.

Receiver Head Unit: scFv
Antirabbit scFV 
on plasmid:
synthesized by BlueHeron
date ordered: July 18
date received: ~2 weeks
[[../Order Confirmation/]]
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