IGEM:MIT/2005/Receiver 1: ToxR

Receiver Device #1 -- ToxR

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Function

To dimerize in the presence of the input signal and cause gene expression to turn on.

Device Depiction

Information path ...

1. Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
2. Fluoroscein binds to adjacent ToxR'-scFv fusions.
   1. Causes ToxR' dimerization
3. GFP expressed @ CTX_promoter
   1. As a function of ToxR dimerization

People path ...

1. Maxine gives us Fluoroscein, or identifies something else
2. Jenny's scFv binds to Maxine's antigen
3. Then, Jenny's scFv induces ToxR' to dimerize
4. The dimerization leads to the expression of Jessica's output @ CTXpromoter [C1, maybe]
5. Ray makes the whole system look like "we meant to do that."

Current Status

In theory this should work (but we need to get the signal into the periplasm)

1. All parts specified
2. But, no parts are ready for synthesis

Open Issues/Questions

• !

1. Can we get the signal in the periplasm?
2. Or, can we adapt or extend the ToxR receiver device so that it accept a signal at the outer membrane.

1. ToxR/scFv Fusion - Can we know with any more certainty that the fusion will work?