IGEM:MIT/2005/Receiver 1: ToxR: Difference between revisions

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==Open Issues/Questions==
==Open Issues/Questions==
* !
* !
#Can we get the signal in the periplasm?
#How will we get our initial test antigens into our cell?
#Or, can we adapt or extend the ToxR receiver device so that it accept a signal at the outer membrane.
#how will we design a system to find more bigger antigen sets?
*
*
#ToxR/scFv Fusion - Can we know with any more certainty that the fusion will work?


==Need Help With==
==Need Help With==

Revision as of 11:57, 13 July 2005

Receiver Device #1 -- ToxR

POC

wiki-Will

Function

To dimerize in the presence of the input signal and cause gene expression to turn on.

Device Depiction

Information path ...

  1. Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
  2. Fluoroscein binds to adjacent ToxR'-scFv fusions.
    1. Causes ToxR' dimerization
  3. GFP expressed @ CTX_promoter
    1. As a function of ToxR dimerization

People path ...

  1. Maxine gives us Fluoroscein, or identifies something else
  2. Jenny's scFv binds to Maxine's antigen
  3. Then, Jenny's scFv induces ToxR' to dimerize
  4. The dimerization leads to the expression of Jessica's output @ CTXpromoter [C1, maybe]
  5. Ray makes the whole system look like "we meant to do that."

Device Parts

Current Status

  1. Going from DNA sequence information to physical genetic material that works inside living cells.
    1. see device parts for what we need ...
  2. Getting Signal Into Periplasm
    1. CAN WE GET OLIGO's AND RUN TESTS NOW ???
  3. All parts specified
    1. and maybe ready for synthesis ...

Open Issues/Questions

  • !
  1. How will we get our initial test antigens into our cell?
  2. how will we design a system to find more bigger antigen sets?

Need Help With

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