IGEM:MIT/2005/Receiver 1: ToxR: Difference between revisions
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==Device Parts== | ==Device Parts== | ||
Composite Parts for the first run of tests. | |||
BBa_J07024 Device ToxR'::scFv Fusion 1 (with promoter and rbs and terminator) 1648 | |||
BBa_J07025 Device ToxR'::scFv Fusion 2 (with promoter and rbs and terminator) 1648 | BBa_J07025 Device ToxR'::scFv Fusion 2 (with promoter and rbs and terminator) 1648 | ||
BBa_J07026 Device ToxR'::scFv Fusion 3 (with promoter and rbs and terminator) 1648 | BBa_J07026 Device ToxR'::scFv Fusion 3 (with promoter and rbs and terminator) 1648 |
Revision as of 11:13, 15 July 2005
Receiver Device #1 -- ToxR
POC
wiki-Will
Function
To cause gene expression in the presence of input signal using the ToxR pathway.
Device Depiction
Information path ...
- Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
- Fluoroscein binds to adjacent transmembrane ToxR'-scFv fusions .
- Causes ToxR' dimerization
- GFP expressed @ CTX_promoter
- As a function of ToxR dimerization
People path ...
- Maxine gives us Fluoroscein, or identifies something else
- Maxine's antigen binds to Jenny's scFv
- Then, Jenny's scFv induces Will's ToxR' to dimerize
- The dimerization leads to the expression of Jessica's output @ CTXpromoter [cI, maybe]
- Ray makes the whole system look like "we meant to do that."
- Jen makes sure it all works.
Device Parts
Composite Parts for the first run of tests. BBa_J07024 Device ToxR'::scFv Fusion 1 (with promoter and rbs and terminator) 1648 BBa_J07025 Device ToxR'::scFv Fusion 2 (with promoter and rbs and terminator) 1648 BBa_J07026 Device ToxR'::scFv Fusion 3 (with promoter and rbs and terminator) 1648 BBa_J07027 Measurement ToxR'::PhoA (with promoter and RBS and terminator) 2300 BBa_J07028 Measurement ToxR'::MalE(with promoter and RBS and terminator) 2075
Current Status
- Ordered all necassary proteins and primers for first synthesis.
- Begin design and outline of necassary system experiments.
- Verify that we can get our signal into the periplasm
- Recieve oligo's 07/15. Lets throw them in our cells
- and if it doesn't "just work," develop and specify how we have to manipulate / weaken our cells to make it work.
- Properly document ordered parts on parts.mit
- Continue research on putting scFv onto outermembrane of cell.
Open Issues/Questions
- The periplasm
- Is the signal going in?
- Are our scFv's going to come out (to the surface)?
- Experimental issues (will = inexperience)
- people tend to be helpful .