IGEM:MIT/2005/Receiver 1: ToxR

Receiver Device #1 -- ToxR

POC

wiki-Will

Function

To cause gene expression in the presence of input signal using the ToxR pathway.

Device Depiction

File:Receiver.1.ToxR e.ppt

Information path ...

1. Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
2. Fluoroscein binds to adjacent transmembrane ToxR'-scFv fusions .
   1. Causes ToxR' dimerization
3. GFP expressed @ CTX_promoter
   1. As a function of ToxR dimerization

People path ...

1. Maxine gives us Fluoroscein, or identifies something else
2. Maxine's antigen binds to Jenny's scFv
3. Then, Jenny's scFv induces Will's ToxR' to dimerize
4. The dimerization leads to the expression of Jessica's output @ CTXpromoter [cI, maybe]
5. Ray makes the whole system look like "we meant to do that."
6. Jen makes sure it all works.

Device Parts

                Composite Parts for the first run of tests. 
                BBa_J07024	Device	ToxR'::scFv Fusion 1 (with promoter and rbs and terminator)	1648	
	  	BBa_J07025	Device	ToxR'::scFv Fusion 2 (with promoter and rbs and terminator)	1648	
	  	BBa_J07026	Device	ToxR'::scFv Fusion 3 (with promoter and rbs and terminator)	1648	
	  	BBa_J07027	Measurement	ToxR'::PhoA (with promoter and RBS and terminator)	2300	
	  	BBa_J07028	Measurement	ToxR'::MalE(with promoter and RBS and terminator)	2075

Current Status

1. Ordered all necassary proteins and primers for first synthesis.
   1. Begin design and outline of necassary system experiments.
2. Verify that we can get our signal into the periplasm
   1. Recieve oligo's 07/15. Lets throw them in our cells
   2. and if it doesn't "just work," develop and specify how we have to manipulate / weaken our cells to make it work.
3. Properly document ordered parts on parts.mit
4. Continue research on putting scFv onto outermembrane of cell.

Open Issues/Questions

1. The periplasm
   1. Is the signal going in?
   2. Are our scFv's going to come out (to the surface)?
2. Experimental issues (will = inexperience)
   1. people tend to be helpful .