IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions
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>Jenny |
>Jenny |
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==Purpose== | ==Purpose== | ||
To provide a landing site for ligand.<br> | To provide a landing site for ligand.<br> | ||
To cause dimerization/conformational change in the Receiver. | To cause dimerization/conformational change in the Receiver.<BR> | ||
To transmit signal to Receiver. | To transmit signal to Receiver. | ||
==Current Status== | ==Current Status== | ||
pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped<br> | pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped<br> | ||
Revision as of 11:17, 12 July 2005
POC
Jenny
Purpose
To provide a landing site for ligand.
To cause dimerization/conformational change in the Receiver.
To transmit signal to Receiver.
Current Status
pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
Trying to design primers to PCR in biobrick format
Open Issues
- TAG (myc) to know that the fluorescein has bound.
- How to design primers so that the sequence is in frame?
Need Help
Open Issues.
