IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
>Jenny No edit summary |
>Jenny |
||
| Line 12: | Line 12: | ||
==Open Issues/Questions== | ==Open Issues/Questions== | ||
*TAG (myc) to know that the fluorescein has bound. | *TAG (myc) to know that the fluorescein has bound. | ||
<s> | *<s>How to design primers so that the sequence is in frame?</s> | ||
==Need Help With== | ==Need Help With== | ||
Check to see if primers are correct. Will talk to Kate 7/13. | Check to see if primers are correct. Will talk to Kate 7/13. | ||
Revision as of 13:41, 12 July 2005
POC
Jenny
Function
- To provide a landing site for ligand.
- To cause dimerization/conformational change in the Receiver.
- To transmit signal to Receiver.
Device Depiction
Device Parts
Current Status
- Primers have been designed for biobrick format.
- pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
Open Issues/Questions
- TAG (myc) to know that the fluorescein has bound.
How to design primers so that the sequence is in frame?
Need Help With
Check to see if primers are correct. Will talk to Kate 7/13.
